BMRB Entry 19308

Name: Biochemical effect of S-67 phosphorylation on UVI31+ from C. reinhardtii
Published: 2014-02-13

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19308

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Biochemical effect of S-67 phosphorylation on UVI31+ from C. reinhardtii

Deposition date:

2013-06-18

Original release date:

2014-02-13

Authors:

Singh, Himanshu; Verma, Deepshikha; Chary, KVR

Citation:

Citation: Singh, Himanshu; Rao, B.; Chary, Kandala. "(1)H, (13)C and (15)N NMR assignments of a mutant of UV inducible transcript (S55A-UVI31+) from Chlamydomonas reinhardtii." Biomol. NMR Assignments ., .-. (2013).
PubMed: 23979961

Assembly members:

Assembly members:
Phophorylated_UVI31+, polymer, 123 residues, 13360 Da.

Natural source:

Natural source: Common Name: Chlamydomonas reinhardtii Taxonomy ID: 3055 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Chlamydomonas reinhardtii

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET and pQE-30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Phophorylated_UVI31+: MRGSHHHHHHGSHVISSIAS RGSMAEHQLGPIAGAIKSKV EAALSPTHFKLINDSHKHAG HYARDGSTASDAGETHFRLE VTSDAFKGLTLVKRHQLIYG LLSDEFKAGLHALSMTTKTP AEQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	321
15N chemical shifts	106
1H chemical shifts	106

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UVI31+	1

Entities:

Entity 1, UVI31+ 123 residues - 13360 Da.

1

MET

ARG

GLY

SER

HIS

2

GLY

SER

HIS

VAL

ILE

SER

ILE

ALA

SER

3

ARG

GLY

SER

MET

ALA

GLU

HIS

GLN

LEU

GLY

4

PRO

ILE

ALA

GLY

ALA

ILE

LYS

SER

LYS

VAL

5

GLU

ALA

LEU

SER

PRO

THR

HIS

PHE

LYS

6

LEU

ILE

ASN

ASP

SER

HIS

LYS

HIS

ALA

GLY

7

HIS

TYR

ALA

ARG

ASP

GLY

SER

THR

ALA

SER

8

ASP

ALA

GLY

GLU

THR

HIS

PHE

ARG

LEU

GLU

9

VAL

THR

SER

ASP

ALA

PHE

LYS

GLY

LEU

THR

10

LEU

VAL

LYS

ARG

HIS

GLN

LEU

ILE

TYR

GLY

11

LEU

SER

ASP

GLU

PHE

LYS

ALA

GLY

LEU

12

HIS

ALA

LEU

SER

MET

THR

LYS

THR

PRO

13

ALA

GLU

GLN

Samples:

sample_1: Phophorylated UVI31+, [U-99% 13C; U-99% 15N], 0.5 mM; phosphate buffer 50 mM; ATP 1.5 mM; MgCl2 5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 100 mM; pH: 6.4; pressure: 1 atm; temperature: 305 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v2, bruker - chemical shift assignment, processing

NMR spectrometers:

Bruker Avance 800 MHz

BMRB	16864 18567 18969 19307
PDB	2MA0
GB	EDO96758
REF	XP_001702905