BMRB Entry 18567

Name: Overexpression, purification and structural characterization of S114A mutant of UVI31+ from chlamydomonas reinhardtii
Published: 2013-02-28

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PDB ID: 2n8q
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18567
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Overexpression, purification and structural characterization of S114A mutant of UVI31+ from chlamydomonas reinhardtii

Deposition date:

2012-07-05

Original release date:

2013-02-28

Authors:

Singh, Himanshu; Chary, Kandala

Citation:

Citation: Singh, Himanshu; Raghavan, Vandana; Shukla, Manish; Rao, Basuthkar; Chary, Kandala. "H, 13C and 15N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii." Biomol. NMR Assignments 8, 71-74 (2014).
PubMed: 23266947

Assembly members:

Assembly members:
S114A_mutant_of_UVI31+, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Green algae Taxonomy ID: 3055 Superkingdom: Eukaryota Kingdom: not available Genus/species: Chlamydomonas reinhardtii

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: chlamydomonas reinhardtii Vector: pET and pQE-30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
S114A_mutant_of_UVI31+: MRGSHHHHHHGSHVISSIAS RGSMAEHQLGPIAGAIKSKV EAALSPTHFKLINDSHKHAG HYARDGSTASDAGETHFRLE VTSDAFKGLTLVKRHQLIYG LLSDEFKAGLHALAMTTKTP AEQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	314
15N chemical shifts	107
1H chemical shifts	107

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	S114A mutant of UVI31+	1

Entities:

Entity 1, S114A mutant of UVI31+ 123 residues - Formula weight is not available

1

MET

ARG

GLY

SER

HIS

2

GLY

SER

HIS

VAL

ILE

SER

ILE

ALA

SER

3

ARG

GLY

SER

MET

ALA

GLU

HIS

GLN

LEU

GLY

4

PRO

ILE

ALA

GLY

ALA

ILE

LYS

SER

LYS

VAL

5

GLU

ALA

LEU

SER

PRO

THR

HIS

PHE

LYS

6

LEU

ILE

ASN

ASP

SER

HIS

LYS

HIS

ALA

GLY

7

HIS

TYR

ALA

ARG

ASP

GLY

SER

THR

ALA

SER

8

ASP

ALA

GLY

GLU

THR

HIS

PHE

ARG

LEU

GLU

9

VAL

THR

SER

ASP

ALA

PHE

LYS

GLY

LEU

THR

10

LEU

VAL

LYS

ARG

HIS

GLN

LEU

ILE

TYR

GLY

11

LEU

SER

ASP

GLU

PHE

LYS

ALA

GLY

LEU

12

HIS

ALA

LEU

ALA

MET

THR

LYS

THR

PRO

13

ALA

GLU

GLN

Samples:

sample_1: S114A mutant of UVI31+, [U-100% 13C; U-100% 15N], 0.8 ± 0.03 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

FELIX v2002, accelrys Inc. - processing

NMR spectrometers:

Bruker Avance 800 MHz

BMRB	18969 19307 19308
PDB	2MA0
GB	EDO96758
REF	XP_001702905