BMRB Entry 19253

Name: Backbone and Cbeta assignments for tau K18 in free and SDS micelle-bound states
Published: 2014-02-13

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19253

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and Cbeta assignments for tau K18 in free and SDS micelle-bound states

Deposition date:

2013-05-21

Original release date:

2014-02-13

Authors:

Eliezer, David; Barr, Patrick

Citation:

Citation: Barre, Patrick; Eliezer, David. "Structural transitions in tau k18 on micelle binding suggest a hierarchy in the efficacy of individual microtubule-binding repeats in filament nucleation." Protein Sci. 22, 1037-1048 (2013).
PubMed: 23740819

Assembly members:

Assembly members:
tau_K18, polymer, 129 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: NA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
tau_K18: QTAPVPMPDLKNVKSKIGST ENLKHQPGGGKVQIINKKLD LSNVQSKCGSKDNIKHVPGG GSVQIVYKPVDLSKVTSKAG SLGNIHHKPGGGQVEVKSEK LDFKDRVQSKIGSLDNITHV PGGGNKKIE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_2

Data type	Count
13C chemical shifts	740
15N chemical shifts	230
1H chemical shifts	230

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	tau_K18	1

Entities:

Entity 1, tau_K18 129 residues - Formula weight is not available

1

GLN

THR

ALA

PRO

VAL

PRO

MET

PRO

ASP

LEU

2

LYS

ASN

VAL

LYS

SER

LYS

ILE

GLY

SER

THR

3

GLU

ASN

LEU

LYS

HIS

GLN

PRO

GLY

4

LYS

VAL

GLN

ILE

ASN

LYS

LEU

ASP

5

LEU

SER

ASN

VAL

GLN

SER

LYS

CYS

GLY

SER

6

LYS

ASP

ASN

ILE

LYS

HIS

VAL

PRO

GLY

7

GLY

SER

VAL

GLN

ILE

VAL

TYR

LYS

PRO

VAL

8

ASP

LEU

SER

LYS

VAL

THR

SER

LYS

ALA

GLY

9

SER

LEU

GLY

ASN

ILE

HIS

LYS

PRO

GLY

10

GLY

GLN

VAL

GLU

VAL

LYS

SER

GLU

LYS

11

LEU

ASP

PHE

LYS

ASP

ARG

VAL

GLN

SER

LYS

12

ILE

GLY

SER

LEU

ASP

ASN

ILE

THR

HIS

VAL

13

PRO

GLY

ASN

LYS

ILE

GLU

Samples:

sample_1: tau_K18, [U-100% 13C; U-100% 15N], 400 – 800 uM; H20 90%; D20 10%

sample_2: tau K18, [U-98% 13C; U-98% 15N], 400 – 800 uM; SDS 100 mM; H20 90%; D20 10%

sample_conditions_1: ionic strength: 160 mM; pH: 7.4; pressure: 1 atm; temperature: 273 K

sample_conditions_2: ionic strength: 160 mM; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_2
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_2
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_2
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_2
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_2	isotropic	sample_conditions_2

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

Bruker DRX 700 MHz
Bruker DRX 800 MHz

BMRB	17920
DBJ	BAE28038
EMBL	CAA32636 CAA55889
GB	AAA30770 AAA42204 AAA51609 AAA58343 AAA58344
REF	NP_001009068 NP_001033698 NP_001104271 NP_001116538 NP_001116539
SP	O02828 P10636 P10637 P19332 P29172
TPE	CAG26750
TPG	DAA18338
AlphaFold	O02828 P10636 P10637 P19332 P29172