BMRB Entry 17920

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17920

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Title:
Assignment of filamentous full-length tau
Deposition date:
2011-09-06
Original release date:
2011-10-24
Authors:
Bibow, Stefan
Citation:

Citation: Bibow, Stefan; Mukrasch, Marco; Chinnathambi, Subashchandrabose; Biernat, Jacek; Griesinger, Christian; Mandelkow, Eckhard; Zweckstetter, Markus. "The dynamic structure of filamentous tau."  Angew. Chem. Int. Ed. Engl. 50, 11520-11524 (2011).
PubMed: 21990182

Assembly members:

Assembly members:
tau-filaments, polymer, 441 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: none

Entity Sequences (FASTA):

Entity Sequences (FASTA):
tau-filaments: MAEPRQEFEVMEDHAGTYGL GDRKDQGGYTMHQDQEGDTD AGLKESPLQTPTEDGSEEPG SETSDAKSTPTAEDVTAPLV DEGAPGKQAAAQPHTEIPEG TTAEEAGIGDTPSLEDEAAG HVTQARMVSKSKDGTGSDDK KAKGADGKTKIATPRGAAPP GQKGQANATRIPAKTPPAPK TPPSSGEPPKSGDRSGYSSP GSPGTPGSRSRTPSLPTPPT REPKKVAVVRTPPKSPSSAK SRLQTAPVPMPDLKNVKSKI GSTENLKHQPGGGKVQIINK KLDLSNVQSKCGSKDNIKHV PGGGSVQIVYKPVDLSKVTS KCGSLGNIHHKPGGGQVEVK SEKLDFKDRVQSKIGSLDNI THVPGGGNKKIETHKLTFRE NAKAKTDHGAEIVYKSPVVS GDTSPRHLSNVSSTGSIDMV DSPQLATLADEVSASLAKQG L

Data sets:
Data typeCount
13C chemical shifts237
15N chemical shifts236
1H chemical shifts216

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1tau-filaments1

Entities:

Entity 1, tau-filaments 441 residues - Formula weight is not available

1   METALAGLUPROARGGLNGLUPHEGLUVAL
2   METGLUASPHISALAGLYTHRTYRGLYLEU
3   GLYASPARGLYSASPGLNGLYGLYTYRTHR
4   METHISGLNASPGLNGLUGLYASPTHRASP
5   ALAGLYLEULYSGLUSERPROLEUGLNTHR
6   PROTHRGLUASPGLYSERGLUGLUPROGLY
7   SERGLUTHRSERASPALALYSSERTHRPRO
8   THRALAGLUASPVALTHRALAPROLEUVAL
9   ASPGLUGLYALAPROGLYLYSGLNALAALA
10   ALAGLNPROHISTHRGLUILEPROGLUGLY
11   THRTHRALAGLUGLUALAGLYILEGLYASP
12   THRPROSERLEUGLUASPGLUALAALAGLY
13   HISVALTHRGLNALAARGMETVALSERLYS
14   SERLYSASPGLYTHRGLYSERASPASPLYS
15   LYSALALYSGLYALAASPGLYLYSTHRLYS
16   ILEALATHRPROARGGLYALAALAPROPRO
17   GLYGLNLYSGLYGLNALAASNALATHRARG
18   ILEPROALALYSTHRPROPROALAPROLYS
19   THRPROPROSERSERGLYGLUPROPROLYS
20   SERGLYASPARGSERGLYTYRSERSERPRO
21   GLYSERPROGLYTHRPROGLYSERARGSER
22   ARGTHRPROSERLEUPROTHRPROPROTHR
23   ARGGLUPROLYSLYSVALALAVALVALARG
24   THRPROPROLYSSERPROSERSERALALYS
25   SERARGLEUGLNTHRALAPROVALPROMET
26   PROASPLEULYSASNVALLYSSERLYSILE
27   GLYSERTHRGLUASNLEULYSHISGLNPRO
28   GLYGLYGLYLYSVALGLNILEILEASNLYS
29   LYSLEUASPLEUSERASNVALGLNSERLYS
30   CYSGLYSERLYSASPASNILELYSHISVAL
31   PROGLYGLYGLYSERVALGLNILEVALTYR
32   LYSPROVALASPLEUSERLYSVALTHRSER
33   LYSCYSGLYSERLEUGLYASNILEHISHIS
34   LYSPROGLYGLYGLYGLNVALGLUVALLYS
35   SERGLULYSLEUASPPHELYSASPARGVAL
36   GLNSERLYSILEGLYSERLEUASPASNILE
37   THRHISVALPROGLYGLYGLYASNLYSLYS
38   ILEGLUTHRHISLYSLEUTHRPHEARGGLU
39   ASNALALYSALALYSTHRASPHISGLYALA
40   GLUILEVALTYRLYSSERPROVALVALSER
41   GLYASPTHRSERPROARGHISLEUSERASN
42   VALSERSERTHRGLYSERILEASPMETVAL
43   ASPSERPROGLNLEUALATHRLEUALAASP
44   GLUVALSERALASERLEUALALYSGLNGLY
45   LEU

Samples:

sample_1: tau-filaments, [U-100% 13C; U-100% 15N], 50 mg; H2O 90%; D2O 10%; Phosphate buffer 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.8; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

GB AAC04279 AAR11260 EAW93567 EAW93569
REF NP_005901 XP_002827442 XP_005257429 XP_005275705 XP_005584597

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks