BMRB Entry 19247

Name: Backbone (1H,15N, CA, CB) assignments of Populus nigra Plastocyanin (Zn)
Published: 2013-06-04

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19247

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone (1H,15N, CA, CB) assignments of Populus nigra Plastocyanin (Zn)

Deposition date:

2013-05-17

Original release date:

2013-06-04

Authors:

Guan, Jia-Ying; Ubbink, Marcellus

Citation:

Citation: Guan, Jia-Ying; Foester, Johannes; Drijfhout, Jan Wouter; Timmer, Monika; Blok, Anneloes; Ullmann, Matthias; Ubbink, Marcellus. "Dynamics between plastocyanin and tetralysine peptides" .

Assembly members:

Assembly members:
plastocyanin, polymer, 101 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Lombardy poplar Taxonomy ID: 3691 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Populus nigra

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
plastocyanin: MGIDVLLGADDGSLAFVPSE FSISPGEKIVFKNNAGFPHN IVFDEDSIPSGVDASKISMS EEDLLNAKGETFEVALSNKG EYSFYCSPHQGAGMVGKVTV N

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_2

Data type	Count
13C chemical shifts	226
15N chemical shifts	114
1H chemical shifts	118

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	plastocyanin	1
2	zinc	2

Entities:

Entity 1, plastocyanin 101 residues - Formula weight is not available

1.The first two residues in the sequence (MG) are not included in the assignment data. The residue numbers in the assignment correspond to the sequence starting from the third residue (I). 2.N-terminal methionine was partially processed and therefore some residues near N-terminus in space have double resonances.

1

MET

GLY

ILE

ASP

VAL

LEU

GLY

ALA

ASP

2

ASP

GLY

SER

LEU

ALA

PHE

VAL

PRO

SER

GLU

3

PHE

SER

ILE

SER

PRO

GLY

GLU

LYS

ILE

VAL

4

PHE

LYS

ASN

ALA

GLY

PHE

PRO

HIS

ASN

5

ILE

VAL

PHE

ASP

GLU

ASP

SER

ILE

PRO

SER

6

GLY

VAL

ASP

ALA

SER

LYS

ILE

SER

MET

SER

7

GLU

ASP

LEU

ASN

ALA

LYS

GLY

GLU

8

THR

PHE

GLU

VAL

ALA

LEU

SER

ASN

LYS

GLY

9

GLU

TYR

SER

PHE

TYR

CYS

SER

PRO

HIS

GLN

10

GLY

ALA

GLY

MET

VAL

GLY

LYS

VAL

THR

VAL

11

ASN

Entity 2, zinc - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: plastocyanin, [U-13C; U-15N], 0.25 ± 0.01 mM; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6.5; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

PDB	5PCY 1JXG 1PLC 1PNC 1PND 1TKW 2PCY 3PCY 4DP7 4DP8 4DP9 4DPA 4DPB 4DPC 4PCY 5PCY 6PCY
BMRB	4019
EMBL	CAA90564
GB	ABK96770 EEE94750
REF	XP_002307754 XP_011003094
SP	P00299
AlphaFold	P00299