BMRB Entry 19247

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19247

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Title:
Backbone (1H,15N, CA, CB) assignments of Populus nigra Plastocyanin (Zn)
Deposition date:
2013-05-17
Original release date:
2013-06-04
Authors:
Guan, Jia-Ying; Ubbink, Marcellus
Citation:

Citation: Guan, Jia-Ying; Foester, Johannes; Drijfhout, Jan Wouter; Timmer, Monika; Blok, Anneloes; Ullmann, Matthias; Ubbink, Marcellus. "Dynamics between plastocyanin and tetralysine peptides"  .

Assembly members:

Assembly members:
plastocyanin, polymer, 101 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Lombardy poplar   Taxonomy ID: 3691   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Populus nigra

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
plastocyanin: MGIDVLLGADDGSLAFVPSE FSISPGEKIVFKNNAGFPHN IVFDEDSIPSGVDASKISMS EEDLLNAKGETFEVALSNKG EYSFYCSPHQGAGMVGKVTV N

Data sets:
Data typeCount
13C chemical shifts226
15N chemical shifts114
1H chemical shifts118

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1plastocyanin1
2zinc2

Entities:

Entity 1, plastocyanin 101 residues - Formula weight is not available

1.The first two residues in the sequence (MG) are not included in the assignment data. The residue numbers in the assignment correspond to the sequence starting from the third residue (I). 2.N-terminal methionine was partially processed and therefore some residues near N-terminus in space have double resonances.

1   METGLYILEASPVALLEULEUGLYALAASP
2   ASPGLYSERLEUALAPHEVALPROSERGLU
3   PHESERILESERPROGLYGLULYSILEVAL
4   PHELYSASNASNALAGLYPHEPROHISASN
5   ILEVALPHEASPGLUASPSERILEPROSER
6   GLYVALASPALASERLYSILESERMETSER
7   GLUGLUASPLEULEUASNALALYSGLYGLU
8   THRPHEGLUVALALALEUSERASNLYSGLY
9   GLUTYRSERPHETYRCYSSERPROHISGLN
10   GLYALAGLYMETVALGLYLYSVALTHRVAL
11   ASN

Entity 2, zinc - Zn - 65.409 Da.

1   ZN

Related Database Links:

PDB 5PCY 1JXG 1PLC 1PNC 1PND 1TKW 2PCY 3PCY 4DP7 4DP8 4DP9 4DPA 4DPB 4DPC 4PCY 6PCY
BMRB 4019
EMBL CAA90564
GB ABK96770 EEE94750
REF XP_002307754 XP_011003094
SP P00299
AlphaFold P00299

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks