BMRB Entry 18667

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18667
MolProbity Validation Chart

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Title:
Solution structure of eIF4E3 in complex with m7GDP
Deposition date:
2012-08-16
Original release date:
2015-07-03
Authors:
Osborne, M; Osborne, Mike; Volpon, L; Kornblatt, J; Culkjovic-kraljcic, B; Borden, A
Citation:

Citation: Osborne, Michael; Volpon, Laurent; Kornblatt, Jack; Culjkovic-kraljcic, Biljana; Baguet, Aurelie; Borden, Katherine. "eIF4E3 acts as a tumor suppressor by utilizing an atypical mode of methyl-7-guanosine cap recognition"  Proc. Natl. Acad. Sci. U. S. A. 110, 3877-3882 (2013).

Assembly members:

Assembly members:
EUKARYOTIC_TRANSLATION_INITIATION_FACTOR_4E_TYPE, polymer, 207 residues, 22831.0043 Da.
7N-METHYL-8-HYDROGUANOSINE-5'-DIPHOSPHATE, non-polymer, 459.243 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: n/a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EUKARYOTIC_TRANSLATION_INITIATION_FACTOR_4E_TYPE: MALPPAAAPPGANEPLDKAL SALPPEPGGVPLHSPWTFWL DRSLPGATAAECASNLKKIY TVQTVQIFWSVYNNIPPVTS LPLRCSYHLMRGERRPLWEE ESNAKGGVWKMKVPKDSTST VWKELLLATIGEQFTDCAAA DDEIIGVSVSVRDREDVVQV WNVNASLVGEATVLEKIHQL LPHIAFKAVFYKPHEEHHAF EGGRGKH

Data sets:
Data typeCount
13C chemical shifts831
15N chemical shifts203
1H chemical shifts1323

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE1
27N-METHYL-8-HYDROGUANOSINE-5'-DIPHOSPHATE2

Entities:

Entity 1, EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE 207 residues - 22831.0043 Da.

1   METALALEUPROPROALAALAALAPROPRO
2   GLYALAASNGLUPROLEUASPLYSALALEU
3   SERALALEUPROPROGLUPROGLYGLYVAL
4   PROLEUHISSERPROTRPTHRPHETRPLEU
5   ASPARGSERLEUPROGLYALATHRALAALA
6   GLUCYSALASERASNLEULYSLYSILETYR
7   THRVALGLNTHRVALGLNILEPHETRPSER
8   VALTYRASNASNILEPROPROVALTHRSER
9   LEUPROLEUARGCYSSERTYRHISLEUMET
10   ARGGLYGLUARGARGPROLEUTRPGLUGLU
11   GLUSERASNALALYSGLYGLYVALTRPLYS
12   METLYSVALPROLYSASPSERTHRSERTHR
13   VALTRPLYSGLULEULEULEUALATHRILE
14   GLYGLUGLNPHETHRASPCYSALAALAALA
15   ASPASPGLUILEILEGLYVALSERVALSER
16   VALARGASPARGGLUASPVALVALGLNVAL
17   TRPASNVALASNALASERLEUVALGLYGLU
18   ALATHRVALLEUGLULYSILEHISGLNLEU
19   LEUPROHISILEALAPHELYSALAVALPHE
20   TYRLYSPROHISGLUGLUHISHISALAPHE
21   GLUGLYGLYARGGLYLYSHIS

Entity 2, 7N-METHYL-8-HYDROGUANOSINE-5'-DIPHOSPHATE - C11 H19 N5 O11 P2 - 459.243 Da.

1   M7G

Samples:

sample_1: EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE, [U-13C; U-15N], 0.25 mM; H2O 90%; D2O, [U-13C; U-15N], 10%

sample_conditions_1: ionic strength: 100.000 mM; pH: 7.200; pressure: 1 atm; temperature: 300.150 K

Experiments:

NameSampleSample stateSample conditions
nasample_1solutionsample_conditions_1

Software:

AutoDep v4.3, PDBe - collection

X-PLOR NIH vany, BRUNGER schwieter - chemical shift assignment

NMR spectrometers:

  • Varian UnityInova 0 MHz

Related Database Links:

UNP IF4E3_MOUSE
BMRB 18663
PDB
DBJ BAB23780 BAB24928
GB AAH27014 AAI68936 AAT45741 EDK99365 EDL91436
REF NP_001100082 NP_080105 XP_005547668 XP_005547669 XP_005547670
SP Q9DBB5
TPG DAA17098
AlphaFold Q3UCK2 Q9DBB5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks