BMRB Entry 18663

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18663
MolProbity Validation Chart

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NMR-STAR v3 text file.
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Title:
The third member of the eIF4E family represses gene expression via a novel mode of recognition of the methyl-7 guanosine cap moiety
Deposition date:
2012-08-15
Original release date:
2013-02-28
Authors:
Osborne, M.; Volpon, Laurent; Kornblatt, J.; Culjkovic-kraljcic, B.; Baguet, A.; Borden, K.; Borden, A.
Citation:

Citation: Osborne, Michael; Volpon, Laurent; Kornblatt, Jack; Culjkovic-Kraljacic, Biljana; Baguet, Aurelie; Borden, Katherine. "eIF4E3 acts as a tumor suppressor by utilizing an atypical mode of methyl-7-guanosine cap recognition."  Proc. Natl. Acad. Sci. U.S.A. 110, 3877-3882 (2013).
PubMed: 23431134

Assembly members:

Assembly members:
EUKARYOTIC_TRANSLATION_INITIATION_FACTOR_4E_TYPE, polymer, 207 residues, 22831.0043 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EUKARYOTIC_TRANSLATION_INITIATION_FACTOR_4E_TYPE: MALPPAAAPPGANEPLDKAL SALPPEPGGVPLHSPWTFWL DRSLPGATAAECASNLKKIY TVQTVQIFWSVYNNIPPVTS LPLRCSYHLMRGERRPLWEE ESNAKGGVWKMKVPKDSTST VWKELLLATIGEQFTDCAAA DDEIIGVSVSVRDREDVVQV WNVNASLVGEATVLEKIHQL LPHIAFKAVFYKPHEEHHAF EGGRGKH

Data sets:
Data typeCount
13C chemical shifts620
15N chemical shifts207
1H chemical shifts1327

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE1

Entities:

Entity 1, EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE 207 residues - 22831.0043 Da.

1   METALALEUPROPROALAALAALAPROPRO
2   GLYALAASNGLUPROLEUASPLYSALALEU
3   SERALALEUPROPROGLUPROGLYGLYVAL
4   PROLEUHISSERPROTRPTHRPHETRPLEU
5   ASPARGSERLEUPROGLYALATHRALAALA
6   GLUCYSALASERASNLEULYSLYSILETYR
7   THRVALGLNTHRVALGLNILEPHETRPSER
8   VALTYRASNASNILEPROPROVALTHRSER
9   LEUPROLEUARGCYSSERTYRHISLEUMET
10   ARGGLYGLUARGARGPROLEUTRPGLUGLU
11   GLUSERASNALALYSGLYGLYVALTRPLYS
12   METLYSVALPROLYSASPSERTHRSERTHR
13   VALTRPLYSGLULEULEULEUALATHRILE
14   GLYGLUGLNPHETHRASPCYSALAALAALA
15   ASPASPGLUILEILEGLYVALSERVALSER
16   VALARGASPARGGLUASPVALVALGLNVAL
17   TRPASNVALASNALASERLEUVALGLYGLU
18   ALATHRVALLEUGLULYSILEHISGLNLEU
19   LEUPROHISILEALAPHELYSALAVALPHE
20   TYRLYSPROHISGLUGLUHISHISALAPHE
21   GLUGLYGLYARGGLYLYSHIS

Samples:

sample_1: EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE, [U-13C; U-15N], 0.2 mM; Phosphate 50 mM; NaCl 100 mM; TCEP 100 uM; D2O, [U-100% 2H], 7%

sample_conditions_1: ionic strength: 100.000 mM; pH: 7.300; pressure: 1.000 atm; temperature: 293.000 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1solutionsample_conditions_1

Software:

AutoDep v4.3, PDBe - collection

SPARKY vany, Goddard - chemical shift assignment

X-PLOR vany, BRUNGER - chemical shift assignment

NMR spectrometers:

  • Varian UnityInova 800 MHz

Related Database Links:

UNP Q9DBB5.1 IF4E3_MOUSE
BMRB 18667
PDB
DBJ BAB23780 BAB24928
GB AAH27014 AAI68936 AAT45741 EDK99365 EDL91436
REF NP_001100082 NP_080105 XP_005547668 XP_005547669 XP_005547670
SP Q9DBB5
TPG DAA17098
AlphaFold Q3UCK2 Q9D983 Q9DBB5

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks