BMRB Entry 18543

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18543

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Title:
Chemical shift assignments of DsbA(C33S) by solid-state NMR
Deposition date:
2012-06-21
Original release date:
2013-04-02
Authors:
Sperling, Lindsay; Tang, Ming; Berthold, Deborah; Nesbitt, Anna; Gennis, Robert; Rienstra, Chad
Citation:

Citation: Sperling, Lindsay; Tang, Ming; Berthold, Deborah; Nesbitt, Anna; Gennis, Robert; Rienstra, Chad. "Solid-State NMR Study of a 41 kDa Membrane Protein Complex DsbA/DsbB."  J. Phys. Chem. B 117, 6052-6060 (2013).
PubMed: 23527473

Assembly members:

Assembly members:
DsbA(C33S), polymer, 189 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Enterobacteria   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE70

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DsbA(C33S): AQYEDGKQYTTLEKPVAGAP QVLEFFSFFCPHSYQFEEVL HISDNVKKKLPEGVKMTKYH VNFMGGDLGKDLTQAWAVAM ALGVEDKVTVPLFEGVQKTQ TIRSASDIRDVFINAGIKGE EYDAAWNSFVVKSLVAQQEK AAADVQLRGVPAMFVNGKYQ LNPQGMDTSNMDVFVQQYAD TVKYLSEKK

Data sets:
Data typeCount
13C chemical shifts518
15N chemical shifts128

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DsbA(C33S)1

Entities:

Entity 1, DsbA(C33S) 189 residues - Formula weight is not available

1   ALAGLNTYRGLUASPGLYLYSGLNTYRTHR
2   THRLEUGLULYSPROVALALAGLYALAPRO
3   GLNVALLEUGLUPHEPHESERPHEPHECYS
4   PROHISSERTYRGLNPHEGLUGLUVALLEU
5   HISILESERASPASNVALLYSLYSLYSLEU
6   PROGLUGLYVALLYSMETTHRLYSTYRHIS
7   VALASNPHEMETGLYGLYASPLEUGLYLYS
8   ASPLEUTHRGLNALATRPALAVALALAMET
9   ALALEUGLYVALGLUASPLYSVALTHRVAL
10   PROLEUPHEGLUGLYVALGLNLYSTHRGLN
11   THRILEARGSERALASERASPILEARGASP
12   VALPHEILEASNALAGLYILELYSGLYGLU
13   GLUTYRASPALAALATRPASNSERPHEVAL
14   VALLYSSERLEUVALALAGLNGLNGLULYS
15   ALAALAALAASPVALGLNLEUARGGLYVAL
16   PROALAMETPHEVALASNGLYLYSTYRGLN
17   LEUASNPROGLNGLYMETASPTHRSERASN
18   METASPVALPHEVALGLNGLNTYRALAASP
19   THRVALLYSTYRLEUSERGLULYSLYS

Samples:

sample_1: DsbA(C33S), [U-100% 13C; U-100% 15N], 15 mg; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 263 K

sample_conditions_2: pH: 7.4; pressure: 1 atm; temperature: 253 K

Experiments:

NameSampleSample stateSample conditions
3D NCACXsample_1solidsample_conditions_1
3D NCOCXsample_1solidsample_conditions_1
2D CCsample_1solidsample_conditions_1
2D TEDORsample_1solidsample_conditions_1

Software:

VNMRJ v3.1, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Varian VXRS 500 MHz

Related Database Links:

BMRB 16327 17710 18396 18544
PDB
DBJ BAB38206 BAE77448 BAG79665 BAI27892 BAI33015
EMBL CAA44868 CAA56736 CAA90910 CAP78318 CAQ34212
GB AAA23715 AAB02995 AAC43519 AAC43520 AAC43521
REF NP_312810 NP_418297 NP_709659 WP_000725331 WP_000725332
SP P0A4L5 P0A4L6 P0AEG4 P0AEG5 P52235
AlphaFold P0A4L5 P0A4L6 P0AEG4 P0AEG5 P52235