BMRB Entry 18396

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18396

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
nanocrystalline DsbA 1H, 13C, 15N chemical shifts
Deposition date:
2012-04-13
Original release date:
2012-09-24
Authors:
Zhou, Donghua
Citation:

Citation: Zhou, Donghua; Nieuwkoop, Andrew; Berthold, Deborah; Comellas, Gemma; Sperling, Lindsay; Tang, Ming; Shah, Gautam; Brea, Elliott; Lemkau, Luisel; Rienstra, Chad. "Solid-state NMR analysis of membrane proteins and protein aggregates by proton detected spectroscopy."  J. Biomol. NMR 54, 291-305 (2012).
PubMed: 22986689

Assembly members:

Assembly members:
oxidized_DsbA, polymer, 189 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSS18

Entity Sequences (FASTA):

Entity Sequences (FASTA):
oxidized_DsbA: AQYEDGKQYTTLEKPVAGAP QVLEFFSFFCPHCYQFEEVL HISDNVKKKLPEGVKMTKYH VNFMGGDLGKDLTQAWAVAM ALGVEDKVTVPLFEGVQKTQ TIRSASDIRDVFINAGIKGE EYDAAWNSFVVKSLVAQQQK AAADVQLRGVPAMFVNGKYQ LNPQGMDTSNMDVFVQQYAD TVKYLSEKK

Data sets:
Data typeCount
13C chemical shifts427
15N chemical shifts138
1H chemical shifts140

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1nanocrystalline DsbA1

Entities:

Entity 1, nanocrystalline DsbA 189 residues - Formula weight is not available

1   ALAGLNTYRGLUASPGLYLYSGLNTYRTHR
2   THRLEUGLULYSPROVALALAGLYALAPRO
3   GLNVALLEUGLUPHEPHESERPHEPHECYS
4   PROHISCYSTYRGLNPHEGLUGLUVALLEU
5   HISILESERASPASNVALLYSLYSLYSLEU
6   PROGLUGLYVALLYSMETTHRLYSTYRHIS
7   VALASNPHEMETGLYGLYASPLEUGLYLYS
8   ASPLEUTHRGLNALATRPALAVALALAMET
9   ALALEUGLYVALGLUASPLYSVALTHRVAL
10   PROLEUPHEGLUGLYVALGLNLYSTHRGLN
11   THRILEARGSERALASERASPILEARGASP
12   VALPHEILEASNALAGLYILELYSGLYGLU
13   GLUTYRASPALAALATRPASNSERPHEVAL
14   VALLYSSERLEUVALALAGLNGLNGLNLYS
15   ALAALAALAASPVALGLNLEUARGGLYVAL
16   PROALAMETPHEVALASNGLYLYSTYRGLN
17   LEUASNPROGLNGLYMETASPTHRSERASN
18   METASPVALPHEVALGLNGLNTYRALAASP
19   THRVALLYSTYRLEUSERGLULYSLYS

Related Database Links:

BMRB 16327 17710 18543 18544
PDB 1A23 1A24 1A2J 1A2L 1A2M 1AC1 1ACV 1BQ7 1DSB 1FVJ 1FVK 1TI1 1U3A 2B3S 2B6M 2HI7 2LEG 2ZUP 3DKS 3E9J 4TKY 4WET 4WEY 4WF4 4WF5
DBJ BAB38206 BAE77448 BAG79665 BAI27892 BAI33015
EMBL CAA44868 CAA56736 CAA90910 CAP78318 CAQ34212
GB AAA23715 AAB02995 AAC43519 AAC43520 AAC43521
REF NP_312810 NP_418297 NP_709659 WP_000725331 WP_000725332
SP P0A4L5 P0A4L6 P0AEG4 P0AEG5 P52235
AlphaFold P0A4L5 P0A4L6 P0AEG4 P0AEG5 P52235

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks