BMRB Entry 18406

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18406

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Title:
Solid-state NMR sequential assignments of Sup35NM
Deposition date:
2012-04-19
Original release date:
2014-02-11
Authors:
Schuetz, Anne; Luckgei, Nina; Habenstein, Birgit; Meier, Beat; Boeckmann, Anja
Citation:

Citation: Luckgei, Nina; Schuetz, Anne; Habenstein, Birgit; Bousset, Luc; Sourigues, Yannick; Melki, Ronald; Meier, Beat; Boeckmann, Anja. "Solid-state NMR sequential assignments of the amyloid core of Sup35pNM"  Biomol. NMR Assign. ., .-. (2013).
PubMed: 23934139

Assembly members:

Assembly members:
Sup35NM, polymer, 273 residues, 30685.8032 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sup35NM: MGSSHHHHHHSSGLVPRGSH MSDSNQGNNQQNYQQYSQNG NQQQGNNRYQGYQAYNAQAQ PAGGYYQNYQGYSGYQQGGY QQYNPDAGYQQQYNPQGGYQ QYNPQGGYQQQFNPQGGRGN YKNFNYNNNLQGYQAGFQPQ SQGMSLNDFQKQQKQAAPKP KKTLKLVSSSGIKLANATKK VGTKPAESDKKEEEKSAETK EPTKEPTKVEEPVKKEEKPV QTEEKTEEKSELPKVEDLKI SESTHNTNNANVTSADALIK EQEEEVDDEVVND

Data sets:
Data typeCount
13C chemical shifts85
15N chemical shifts31

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sup35NM1

Entities:

Entity 1, Sup35NM 273 residues - 30685.8032 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METSERASPSERASNGLNGLYASNASNGLN
4   GLNASNTYRGLNGLNTYRSERGLNASNGLY
5   ASNGLNGLNGLNGLYASNASNARGTYRGLN
6   GLYTYRGLNALATYRASNALAGLNALAGLN
7   PROALAGLYGLYTYRTYRGLNASNTYRGLN
8   GLYTYRSERGLYTYRGLNGLNGLYGLYTYR
9   GLNGLNTYRASNPROASPALAGLYTYRGLN
10   GLNGLNTYRASNPROGLNGLYGLYTYRGLN
11   GLNTYRASNPROGLNGLYGLYTYRGLNGLN
12   GLNPHEASNPROGLNGLYGLYARGGLYASN
13   TYRLYSASNPHEASNTYRASNASNASNLEU
14   GLNGLYTYRGLNALAGLYPHEGLNPROGLN
15   SERGLNGLYMETSERLEUASNASPPHEGLN
16   LYSGLNGLNLYSGLNALAALAPROLYSPRO
17   LYSLYSTHRLEULYSLEUVALSERSERSER
18   GLYILELYSLEUALAASNALATHRLYSLYS
19   VALGLYTHRLYSPROALAGLUSERASPLYS
20   LYSGLUGLUGLULYSSERALAGLUTHRLYS
21   GLUPROTHRLYSGLUPROTHRLYSVALGLU
22   GLUPROVALLYSLYSGLUGLULYSPROVAL
23   GLNTHRGLUGLULYSTHRGLUGLULYSSER
24   GLULEUPROLYSVALGLUASPLEULYSILE
25   SERGLUSERTHRHISASNTHRASNASNALA
26   ASNVALTHRSERALAASPALALEUILELYS
27   GLUGLNGLUGLUGLUVALASPASPGLUVAL
28   VALASNASP

Samples:

Sup35NM_1: Sup35NM, [U-100% 13C; U-100% 15N], 15 ± 2 mg

Condition: ionic strength: 0 M; pH: 7.000; pressure: 1.000 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
DARR 20 msSup35NM_1solidCondition
NCASup35NM_1solidCondition
NCACBSup35NM_1solidCondition
CANCOSup35NM_1solidCondition
NCOCXSup35NM_1solidCondition
NCACOSup35NM_1solidCondition
DARR 100 msSup35NM_1solidCondition
NCOCASup35NM_1solidCondition
NCACXSup35NM_1solidCondition
CCCSup35NM_1solidCondition
HC INEPTSup35NM_1solidCondition
NCOSup35NM_1solidCondition

Software:

ANALYSIS v2.1, CCPN - chemical shift assignment

CcpNMR vany, CCPN - Spectrum analysis, Spectrum display

NMR spectrometers:

  • Bruker Avance 850 MHz

Related Database Links:

BMRB 15379 18407
DBJ GAA22402
EMBL CAA30155 CAA68760 CAA86677 CAY78675
GB AAA35133 AAF99684 AAK26176 AAK26180 AAK26182
REF NP_010457
SP P05453
TPG DAA12014
AlphaFold P05453