BMRB Entry 18407

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18407

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Title:
Solid-state NMR sequential assignments of full-length Sup35p
Deposition date:
2012-04-19
Original release date:
2014-02-11
Authors:
Schuetz, Anne; Luckgei, Nina; Habenstein, Birgit; Meier, Beat; Boeckmann, Anja
Citation:

Citation: Schuetz, Anne; Habenstein, Birgit; Luckgei, Nina; Bousset, Luc; Sourigues, Yannick; Nielsen, Anders; Melki, Ronald; Boeckmann, Anja; Meier, Beat. "Solid-state NMR sequential assignments of the amyloid core of full-length Sup35p"  Biomol. NMR Assign. ., .-. (2013).
PubMed: 23943018

Assembly members:

Assembly members:
Sup35, polymer, 705 residues, 78732.142 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sup35: MGSSHHHHHHSSGLVPRGSH MSDSNQGNNQQNYQQYSQNG NQQQGNNRYQGYQAYNAQAQ PAGGYYQNYQGYSGYQQGGY QQYNPDAGYQQQYNPQGGYQ QYNPQGGYQQQFNPQGGRGN YKNFNYNNNLQGYQAGFQPQ SQGMSLNDFQKQQKQAAPKP KKTLKLVSSSGIKLANATKK VGTKPAESDKKEEEKSAETK EPTKEPTKVEEPVKKEEKPV QTEEKTEEKSELPKVEDLKI SESTHNTNNANVTSADALIK EQEEEVDDEVVNDMFGGKDH VSLIFMGHVDAGKSTMGGNL LYLTGSVDKRTIEKYEREAK DAGRQGWYLSWVMDTNKEER NDGKTIEVGKAYFETEKRRY TILDAPGHKMYVSEMIGGAS QADVGVLVISARKGEYETGF ERGGQTREHALLAKTQGVNK MVVVVNKMDDPTVNWSKERY DQCVSNVSNFLRAIGYNIKT DVVFMPVSGYSGANLKDHVD PKECPWYTGPTLLEYLDTMN HVDRHINAPFMLPIAAKMKD LGTIVEGKIESGHIKKGQST LLMPNKTAVEIQNIYNETEN EVDMAMCGEQVKLRIKGVEE EDISPGFVLTSPKNPIKSVT KFVAQIAIVELKSIIAAGFS CVMHVHTAIEEVHIVKLLHK LEKGTNRKSKKPPAFAKKGM KVIAVLETEAPVCVETYQDY PQLGRFTLRDQGTTIAIGKI VKIAE

Data sets:
Data typeCount
13C chemical shifts84
15N chemical shifts32

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Sup351

Entities:

Entity 1, Sup35 705 residues - 78732.142 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METSERASPSERASNGLNGLYASNASNGLN
4   GLNASNTYRGLNGLNTYRSERGLNASNGLY
5   ASNGLNGLNGLNGLYASNASNARGTYRGLN
6   GLYTYRGLNALATYRASNALAGLNALAGLN
7   PROALAGLYGLYTYRTYRGLNASNTYRGLN
8   GLYTYRSERGLYTYRGLNGLNGLYGLYTYR
9   GLNGLNTYRASNPROASPALAGLYTYRGLN
10   GLNGLNTYRASNPROGLNGLYGLYTYRGLN
11   GLNTYRASNPROGLNGLYGLYTYRGLNGLN
12   GLNPHEASNPROGLNGLYGLYARGGLYASN
13   TYRLYSASNPHEASNTYRASNASNASNLEU
14   GLNGLYTYRGLNALAGLYPHEGLNPROGLN
15   SERGLNGLYMETSERLEUASNASPPHEGLN
16   LYSGLNGLNLYSGLNALAALAPROLYSPRO
17   LYSLYSTHRLEULYSLEUVALSERSERSER
18   GLYILELYSLEUALAASNALATHRLYSLYS
19   VALGLYTHRLYSPROALAGLUSERASPLYS
20   LYSGLUGLUGLULYSSERALAGLUTHRLYS
21   GLUPROTHRLYSGLUPROTHRLYSVALGLU
22   GLUPROVALLYSLYSGLUGLULYSPROVAL
23   GLNTHRGLUGLULYSTHRGLUGLULYSSER
24   GLULEUPROLYSVALGLUASPLEULYSILE
25   SERGLUSERTHRHISASNTHRASNASNALA
26   ASNVALTHRSERALAASPALALEUILELYS
27   GLUGLNGLUGLUGLUVALASPASPGLUVAL
28   VALASNASPMETPHEGLYGLYLYSASPHIS
29   VALSERLEUILEPHEMETGLYHISVALASP
30   ALAGLYLYSSERTHRMETGLYGLYASNLEU
31   LEUTYRLEUTHRGLYSERVALASPLYSARG
32   THRILEGLULYSTYRGLUARGGLUALALYS
33   ASPALAGLYARGGLNGLYTRPTYRLEUSER
34   TRPVALMETASPTHRASNLYSGLUGLUARG
35   ASNASPGLYLYSTHRILEGLUVALGLYLYS
36   ALATYRPHEGLUTHRGLULYSARGARGTYR
37   THRILELEUASPALAPROGLYHISLYSMET
38   TYRVALSERGLUMETILEGLYGLYALASER
39   GLNALAASPVALGLYVALLEUVALILESER
40   ALAARGLYSGLYGLUTYRGLUTHRGLYPHE
41   GLUARGGLYGLYGLNTHRARGGLUHISALA
42   LEULEUALALYSTHRGLNGLYVALASNLYS
43   METVALVALVALVALASNLYSMETASPASP
44   PROTHRVALASNTRPSERLYSGLUARGTYR
45   ASPGLNCYSVALSERASNVALSERASNPHE
46   LEUARGALAILEGLYTYRASNILELYSTHR
47   ASPVALVALPHEMETPROVALSERGLYTYR
48   SERGLYALAASNLEULYSASPHISVALASP
49   PROLYSGLUCYSPROTRPTYRTHRGLYPRO
50   THRLEULEUGLUTYRLEUASPTHRMETASN
51   HISVALASPARGHISILEASNALAPROPHE
52   METLEUPROILEALAALALYSMETLYSASP
53   LEUGLYTHRILEVALGLUGLYLYSILEGLU
54   SERGLYHISILELYSLYSGLYGLNSERTHR
55   LEULEUMETPROASNLYSTHRALAVALGLU
56   ILEGLNASNILETYRASNGLUTHRGLUASN
57   GLUVALASPMETALAMETCYSGLYGLUGLN
58   VALLYSLEUARGILELYSGLYVALGLUGLU
59   GLUASPILESERPROGLYPHEVALLEUTHR
60   SERPROLYSASNPROILELYSSERVALTHR
61   LYSPHEVALALAGLNILEALAILEVALGLU
62   LEULYSSERILEILEALAALAGLYPHESER
63   CYSVALMETHISVALHISTHRALAILEGLU
64   GLUVALHISILEVALLYSLEULEUHISLYS
65   LEUGLULYSGLYTHRASNARGLYSSERLYS
66   LYSPROPROALAPHEALALYSLYSGLYMET
67   LYSVALILEALAVALLEUGLUTHRGLUALA
68   PROVALCYSVALGLUTHRTYRGLNASPTYR
69   PROGLNLEUGLYARGPHETHRLEUARGASP
70   GLNGLYTHRTHRILEALAILEGLYLYSILE
71   VALLYSILEALAGLU

Samples:

Sup35p: Sup35, [U-100% 13C; U-100% 15N], 15 ± 2 mg

Condition: ionic strength: 0 M; pH: 7.000; pressure: 1.000 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
DARR 100 msSup35psolidCondition
DARR 20msSup35psolidCondition
NCASup35psolidCondition
NCACBSup35psolidCondition
NCOCASup35psolidCondition
HC INEPTSup35psolidCondition
NCACOSup35psolidCondition
CCCSup35psolidCondition
NCACXSup35psolidCondition
NCOSup35psolidCondition
NCOCXSup35psolidCondition
CANCOSup35psolidCondition
DREAMSup35psolidCondition

Software:

ANALYSIS v2.1, CCPN - chemical shift assignment

CcpNMR vany, CCPN - Spectrum analysis, Spectrum display

NMR spectrometers:

  • Bruker Avance 850 MHz

Related Database Links:

PDB
DBJ GAA22402
EMBL CAA30155 CAA68760 CAA86677 CAY78675
GB AAA35133 AAF99684 AAK26176 AAK26180 AAL15028
REF NP_010457
SP P05453
TPG DAA12014
AlphaFold P05453