BMRB Entry 18397
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18397
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Citation: Zhou, Donghua; Nieuwkoop, Andrew; Berthold, Deborah; Comellas, Gemma; Sperling, Lindsay; Tang, Ming; Shah, Gautam; Brea, Elliott; Lemkau, Luisel; Rienstra, Chad. "Solid-state NMR analysis of membrane proteins and protein aggregates by proton detected spectroscopy." J. Biomol. NMR 54, 291-305 (2012).
PubMed: 22986689
Assembly members:
GB1, polymer, 56 residues, Formula weight is not available
Natural source: Common Name: Streptococcal bacteria Taxonomy ID: 1301 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptococcal bacteria
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: NA
Entity Sequences (FASTA):
GB1: MQYKLILNGKTLKGETTTEA
VDAATAEKVFKQYANDNGVD
GEWTYDDATKTFTVTE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 186 |
| 15N chemical shifts | 61 |
| 1H chemical shifts | 66 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GB1 | 1 |
Entities:
Entity 1, GB1 56 residues - Formula weight is not available
| 1 | MET | GLN | TYR | LYS | LEU | ILE | LEU | ASN | GLY | LYS | ||||
| 2 | THR | LEU | LYS | GLY | GLU | THR | THR | THR | GLU | ALA | ||||
| 3 | VAL | ASP | ALA | ALA | THR | ALA | GLU | LYS | VAL | PHE | ||||
| 4 | LYS | GLN | TYR | ALA | ASN | ASP | ASN | GLY | VAL | ASP | ||||
| 5 | GLY | GLU | TRP | THR | TYR | ASP | ASP | ALA | THR | LYS | ||||
| 6 | THR | PHE | THR | VAL | THR | GLU |
Samples:
sample_1: GB1, [U-98% 13C; U-98% 15N], 5 mg
sample_conditions_1: pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NH | sample_1 | solid | sample_conditions_1 |
| CANH | sample_1 | solid | sample_conditions_1 |
| CA(CO)NH | sample_1 | solid | sample_conditions_1 |
| CANH | sample_1 | solid | sample_conditions_1 |
| CONH | sample_1 | solid | sample_conditions_1 |
| CO(CA)NH | sample_1 | solid | sample_conditions_1 |
| CBCANH | sample_1 | solid | sample_conditions_1 |
| CBCA(CO)NH | sample_1 | solid | sample_conditions_1 |
Software:
SPARKY, Goddard - data analysis
NMR spectrometers:
- Varian INOVA 750 MHz
Related Database Links:
| BMRB | 15156 15380 16444 16627 16755 16873 16882 16958 17810 19394 26630 |
| PDB | |
| EMBL | CAA37410 |
| GB | AAY41168 |
Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone
or all simulated peaks