BMRB Entry 26630

Name: Protein G Domain Beta-1 Wild Type
Published: 2015-09-29

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26630

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Protein G Domain Beta-1 Wild Type

Deposition date:

2015-08-10

Original release date:

2015-09-29

Authors:

Davey, James; Damry, Adam; Euler, Christian; Goto, Natalie; Chica, Roberto

Citation:

Citation: Davey, James; Damry, Adam; Euler, Christian; Goto, Natalie; Chica, Roberto. "Prediction of Stable Globular Proteins Using Negative Design with Non-native Backbone Ensembles" Structure 23, 2011-2021 (2015).
PubMed: 26412333

Assembly members:

Assembly members:
Protein_G_Domain_Beta-1_Wild_Type, polymer, 64 residues, Formula weight is not available

Natural source:

Natural source: Common Name: firmicutes Taxonomy ID: 1325 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptococcus not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pJ414

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Protein_G_Domain_Beta-1_Wild_Type: MHHHHHHGMTYKLILNGKTL KGETTTEAVDAATAEKVFKQ YANDNGVDGEWTYDDATKTF TVTE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	165
15N chemical shifts	56
1H chemical shifts	56

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Protein G Domain Beta-1 Wild-Type	1

Entities:

Entity 1, Protein G Domain Beta-1 Wild-Type 64 residues - Formula weight is not available

1

MET

HIS

GLY

MET

THR

2

TYR

LYS

LEU

ILE

LEU

ASN

GLY

LYS

THR

LEU

3

LYS

GLY

GLU

THR

GLU

ALA

VAL

ASP

4

ALA

THR

ALA

GLU

LYS

VAL

PHE

LYS

GLN

5

TYR

ALA

ASN

ASP

ASN

GLY

VAL

ASP

GLY

GLU

6

TRP

THR

TYR

ASP

ALA

THR

LYS

THR

PHE

7

THR

VAL

THR

GLU

Samples:

sample_1: Protein G Domain Beta-1 Wild Type, [U-99% 13C; U-98% 15N], 0.5 – 2 mM; D2O, [U-99.9% 2H], 10%; H2O 90%; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7.4; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Varian INOVA 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks