BMRB Entry 18307

Name: Human APOBEC2 chemical shifts
Published: 2012-03-28

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18307

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Human APOBEC2 chemical shifts

Deposition date:

2012-03-01

Original release date:

2012-03-28

Authors:

Krzysiak, Troy; Jung, Jinwon; Thompson, James; Baker, David; Gronenborn, Angela

Citation:

Citation: Krzysiak, Troy; Jung, Jinwon; Thompson, James; Baker, David; Gronenborn, Angela. "APOBEC2 is a Monomer in Solution: Implications for APOBEC3G Models" Biochemistry 51, 2008-2017 (2012).
PubMed: 22339232

Assembly members:

Assembly members:
APOBEC2_1-224, polymer, 227 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET41a modified

Entity Sequences (FASTA):

Entity Sequences (FASTA):
APOBEC2_1-224: SEFMAQKEEAAVATEAASQN GEDLENLDDPEKLKELIELP PFEIVTGERLPANFFKFQFR NVEYSSGRNKTFLCYVVEAQ GKGGQVQASRGYLEDEHAAA HAEEAFFNTILPAFDPALRY NVTWYVSSSPCAACADRIIK TLSKTKNLRLLILVGRLFMW EEPEIQAALKKLKEAGCKLR IMKPQDFEYVWQNFVEQEEG ESKAFQPWEDIQENFLYYEE KLADILK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	387
15N chemical shifts	198
1H chemical shifts	198

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	A2POBEC2 1-224	1

Entities:

Entity 1, A2POBEC2 1-224 227 residues - Formula weight is not available

Start SEF are cloning artifacts

1

SER

GLU

PHE

MET

ALA

GLN

LYS

GLU

ALA

2

ALA

VAL

ALA

THR

GLU

ALA

SER

GLN

ASN

3

GLY

GLU

ASP

LEU

GLU

ASN

LEU

ASP

PRO

4

GLU

LYS

LEU

LYS

GLU

LEU

ILE

GLU

LEU

PRO

5

PRO

PHE

GLU

ILE

VAL

THR

GLY

GLU

ARG

LEU

6

PRO

ALA

ASN

PHE

LYS

PHE

GLN

PHE

ARG

7

ASN

VAL

GLU

TYR

SER

GLY

ARG

ASN

LYS

8

THR

PHE

LEU

CYS

TYR

VAL

GLU

ALA

GLN

9

GLY

LYS

GLY

GLN

VAL

GLN

ALA

SER

ARG

10

GLY

TYR

LEU

GLU

ASP

GLU

HIS

ALA

11

HIS

ALA

GLU

ALA

PHE

ASN

THR

ILE

12

LEU

PRO

ALA

PHE

ASP

PRO

ALA

LEU

ARG

TYR

13

ASN

VAL

THR

TRP

TYR

VAL

SER

PRO

14

CYS

ALA

CYS

ALA

ASP

ARG

ILE

LYS

15

THR

LEU

SER

LYS

THR

LYS

ASN

LEU

ARG

LEU

16

LEU

ILE

LEU

VAL

GLY

ARG

LEU

PHE

MET

TRP

17

GLU

PRO

GLU

ILE

GLN

ALA

LEU

LYS

18

LYS

LEU

LYS

GLU

ALA

GLY

CYS

LYS

LEU

ARG

19

ILE

MET

LYS

PRO

GLN

ASP

PHE

GLU

TYR

VAL

20

TRP

GLN

ASN

PHE

VAL

GLU

GLN

GLU

GLY

21

GLU

SER

LYS

ALA

PHE

GLN

PRO

TRP

GLU

ASP

22

ILE

GLN

GLU

ASN

PHE

LEU

TYR

GLU

23

LYS

LEU

ALA

ASP

ILE

LEU

LYS

Samples:

sample_1: HEPES 20 mM; sodium chloride 50 mM; DTT 5 mM; APOBEC2 1-224, [U-100% 13C; U-100% 15N; U-80% 2H], 1 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 700 MHz

BMRB	18306
PDB	2NYT
DBJ	BAD97181 BAG36096
EMBL	CAH90732
GB	AAD45360 AAH47767 AAH69688 AAH69764 AAT44388
REF	NP_001125408 NP_006780 XP_001117195 XP_003266357 XP_003897628
SP	Q694B4 Q9Y235
AlphaFold	Q694B4 Q9Y235