BMRB Entry 18306

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18306

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Title:
Human APOBEC2 chemical shifts, RDC, NOE, and T1/T2 data
Deposition date:
2012-03-01
Original release date:
2012-03-28
Authors:
Krzysiak, Troy; Jung, Jinwon; Thompson, James; Baker, David; Gronenborn, Angela
Citation:

Citation: Krzysiak, Troy; Jung, Jinwon; Thompson, James; Baker, David; Gronenborn, Angela. "APOBEC2 is a Monomer in Solution: Implications for APOBEC3G Models"  Biochemistry 51, 2008-2017 (2012).
PubMed: 22339232

Assembly members:

Assembly members:
APOBEC2_41-224, polymer, 187 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET41a modified

Entity Sequences (FASTA):

Entity Sequences (FASTA):
APOBEC2_41-224: SEFIVTGERLPANFFKFQFR NVEYSSGRNKTFLCYVVEAQ GKGGQVQASRGYLEDEHAAA HAEEAFFNTILPAFDPALRY NVTWYVSSSPCAACADRIIK TLSKTKNLRLLILVGRLFMW EEPEIQAALKKLKEAGCKLR IMKPQDFEYVWQNFVEQEEG ESKAFQPWEDIQENFLYYEE KLADILK

Data sets:
Data typeCount
13C chemical shifts466
15N chemical shifts158
1H chemical shifts158
heteronuclear NOE values152
residual dipolar couplings304
T1 relaxation values108
T2 relaxation values108

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1A2POBEC2 41-2241

Entities:

Entity 1, A2POBEC2 41-224 187 residues - Formula weight is not available

Start SEF are cloning artifacts

1   SERGLUPHEILEVALTHRGLYGLUARGLEU
2   PROALAASNPHEPHELYSPHEGLNPHEARG
3   ASNVALGLUTYRSERSERGLYARGASNLYS
4   THRPHELEUCYSTYRVALVALGLUALAGLN
5   GLYLYSGLYGLYGLNVALGLNALASERARG
6   GLYTYRLEUGLUASPGLUHISALAALAALA
7   HISALAGLUGLUALAPHEPHEASNTHRILE
8   LEUPROALAPHEASPPROALALEUARGTYR
9   ASNVALTHRTRPTYRVALSERSERSERPRO
10   CYSALAALACYSALAASPARGILEILELYS
11   THRLEUSERLYSTHRLYSASNLEUARGLEU
12   LEUILELEUVALGLYARGLEUPHEMETTRP
13   GLUGLUPROGLUILEGLNALAALALEULYS
14   LYSLEULYSGLUALAGLYCYSLYSLEUARG
15   ILEMETLYSPROGLNASPPHEGLUTYRVAL
16   TRPGLNASNPHEVALGLUGLNGLUGLUGLY
17   GLUSERLYSALAPHEGLNPROTRPGLUASP
18   ILEGLNGLUASNPHELEUTYRTYRGLUGLU
19   LYSLEUALAASPILELEULYS

Related Database Links:

BMRB 18307
PDB 2NYT
DBJ BAD97181 BAG36096
EMBL CAH90732
GB AAD45360 AAH47767 AAH69688 AAH69764 AAT44388
REF NP_001125408 NP_006780 XP_001117195 XP_002746566 XP_003266357
SP Q694B4 Q9Y235
AlphaFold Q694B4 Q9Y235

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks