BMRB Entry 18293

Name: Backbone 1HN, 13C, and 15N Chemical Shift Assignments for Granulocyte colony-stimulating factor A29G
Published: 2012-08-21

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18293

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1HN, 13C, and 15N Chemical Shift Assignments for Granulocyte colony-stimulating factor A29G

Deposition date:

2012-02-24

Original release date:

2012-08-21

Authors:

Sauve, Simon; Hodgson, Derek; Aubin, Yves

Citation:

Citation: Hodgson, Derek; Thach, William; Gingras, Genevieve; Sauve, Simon; Bristow, Adrian; Aubin, Yves. "Comparability Study of the Structure of Filgrastim and Mutants using the NMR Fingerprint Assay" J. Pharm. Sci. ., .-..

Assembly members:

Assembly members:
metGCSFA30G, polymer, 175 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b10

Entity Sequences (FASTA):

Entity Sequences (FASTA):
metGCSFA30G: MTPLGPASSLPQSFLLKCLE QVRKIQGDGGALQEKLCATY KLCHPEELVLLGHSLGIPWA PLSSCPSQALQLAGCLSQLH SGLFLYQGLLQALEGISPEL GPTLDTLQLDVADFATTIWQ QMEELGMAPALQPTQGAMPA FASAFQRRAGGVLVASHLQS FLEVSYRVLRHLAQP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	476
15N chemical shifts	156
1H chemical shifts	159

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Granulocyte colony-stimulating factor A29G	1

Entities:

Entity 1, Granulocyte colony-stimulating factor A29G 175 residues - Formula weight is not available

Residue 1 is a methionine Residue 30 is mutated from Ala to Gly

1

MET

THR

PRO

LEU

GLY

PRO

ALA

SER

LEU

2

PRO

GLN

SER

PHE

LEU

LYS

CYS

LEU

GLU

3

GLN

VAL

ARG

LYS

ILE

GLN

GLY

ASP

GLY

4

ALA

LEU

GLN

GLU

LYS

LEU

CYS

ALA

THR

TYR

5

LYS

LEU

CYS

HIS

PRO

GLU

LEU

VAL

LEU

6

LEU

GLY

HIS

SER

LEU

GLY

ILE

PRO

TRP

ALA

7

PRO

LEU

SER

CYS

PRO

SER

GLN

ALA

LEU

8

GLN

LEU

ALA

GLY

CYS

LEU

SER

GLN

LEU

HIS

9

SER

GLY

LEU

PHE

LEU

TYR

GLN

GLY

LEU

10

GLN

ALA

LEU

GLU

GLY

ILE

SER

PRO

GLU

LEU

11

GLY

PRO

THR

LEU

ASP

THR

LEU

GLN

LEU

ASP

12

VAL

ALA

ASP

PHE

ALA

THR

ILE

TRP

GLN

13

GLN

MET

GLU

LEU

GLY

MET

ALA

PRO

ALA

14

LEU

GLN

PRO

THR

GLN

GLY

ALA

MET

PRO

ALA

15

PHE

ALA

SER

ALA

PHE

GLN

ARG

ALA

GLY

16

GLY

VAL

LEU

VAL

ALA

SER

HIS

LEU

GLN

SER

17

PHE

LEU

GLU

VAL

SER

TYR

ARG

VAL

LEU

ARG

18

HIS

LEU

ALA

GLN

PRO

Samples:

sample_1: metGCSFA30G, [U-99% 13C; U-99% 15N], 1 mM; sodium acetate 25 mM; D2O 5%; H2O 95%

sample_conditions_1: pH: 4.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection

NMRView v8.2.15, Johnson, One Moon Scientific - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance III 600 MHz

BMRB	18291 18294 18295
PDB	1CD9 1GNC 1PGR 1RHG 2D9Q
DBJ	BAF82936 BAG36289 BAJ21174
EMBL	CAA27168 CAA27290 CAA27291 CAG46689
GB	AAA03056 AAA35882 AAH33245 AAK62469 AAV38741
PRF	1203374A
REF	NP_000750 NP_757373 XP_002827665 XP_003278307 XP_003831483
SP	P09919
AlphaFold	P09919