BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18291

Title: Backbone 1HN, 13C, and 15N Chemical Shift Assignments for Granulocyte colony-stimulating factor

Deposition date: 2012-02-23 Original release date: 2012-08-21

Authors: Sauve, Simon; Hodgson, Derek; Aubin, Yves

Citation: Hodgson, Derek; Thach, William; Gingras, Genevieve; Sauve, Simon; Bristow, Adrian; Aubin, Yves. "Comparability Study of the Structure of Filgrastim and Mutants using the NMR Fingerprint Assay"  J. Pharm. Sci. ., .-..

Assembly members:
metGCSF, polymer, 175 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b10

Entity Sequences (FASTA):
metGCSF: MTPLGPASSLPQSFLLKCLE QVRKIQGDGAALQEKLCATY KLCHPEELVLLGHSLGIPWA PLSSCPSQALQLAGCLSQLH SGLFLYQGLLQALEGISPEL GPTLDTLQLDVADFATTIWQ QMEELGMAPALQPTQGAMPA FASAFQRRAGGVLVASHLQS FLEVSYRVLRHLAQP

Data sets:
Data typeCount
13C chemical shifts475
15N chemical shifts155
1H chemical shifts157

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Granulocyte colony-stimulating factor1

Entities:

Entity 1, Granulocyte colony-stimulating factor 175 residues - Formula weight is not available

Residue 1 is a methionine

1   METTHRPROLEUGLYPROALASERSERLEU
2   PROGLNSERPHELEULEULYSCYSLEUGLU
3   GLNVALARGLYSILEGLNGLYASPGLYALA
4   ALALEUGLNGLULYSLEUCYSALATHRTYR
5   LYSLEUCYSHISPROGLUGLULEUVALLEU
6   LEUGLYHISSERLEUGLYILEPROTRPALA
7   PROLEUSERSERCYSPROSERGLNALALEU
8   GLNLEUALAGLYCYSLEUSERGLNLEUHIS
9   SERGLYLEUPHELEUTYRGLNGLYLEULEU
10   GLNALALEUGLUGLYILESERPROGLULEU
11   GLYPROTHRLEUASPTHRLEUGLNLEUASP
12   VALALAASPPHEALATHRTHRILETRPGLN
13   GLNMETGLUGLULEUGLYMETALAPROALA
14   LEUGLNPROTHRGLNGLYALAMETPROALA
15   PHEALASERALAPHEGLNARGARGALAGLY
16   GLYVALLEUVALALASERHISLEUGLNSER
17   PHELEUGLUVALSERTYRARGVALLEUARG
18   HISLEUALAGLNPRO

Samples:

sample_1: metGCSF, [U-99% 13C; U-99% 15N], 1 mM; sodium acetate 25 mM; D2O 5%; H2O 95%

sample_conditions_1: pH: 4.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection

NMRView v8.2.15, Johnson, One Moon Scientific - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance III 600 MHz

Related Database Links:

BMRB 18293 18294 18295
PDB
DBJ BAF82936 BAG36289 BAJ21174
EMBL CAA27168 CAA27290 CAA27291 CAG46689
GB AAA03056 AAA35882 AAH33245 AAK62469 AAV38741
PRF 1203374A
REF NP_000750 NP_757373 XP_002827665 XP_003278307 XP_003831483
SP P09919

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts