BMRB Entry 18106

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18106

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Title:
MARCKS MH2 Domain free and mAb 3C3 bounded
Deposition date:
2011-11-28
Original release date:
2014-03-14
Authors:
Tinoco, Luzineide; Fraga, Jully; AnoBom, Cristiane; Zolessi, Flavio; Obal, Gonzalo; Toledo, Andrea; Pritsch, Otto; Arruti, Cristina
Citation:

Citation: Tinoco, Luzineide; Fraga, Jully; AnoBom, Cristiane; Zolessi, Flavio; Obal, Gonzalo; Toledo, Andrea; Pritsch, Otto; Arruti, Cristina. "Structural characterization of a neuroblast-specific phosphorylated region of MARCKS"  Biochim. Biophys. Acta 1844, 837-849 (2014).
PubMed: 24590112

Assembly members:

Assembly members:
MARCKS_peptides, polymer, 20 residues, 1941 Da.
3C3, non-polymer, 281.275 Da.

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MARCKS_peptides: EKPGEAVAAXPSKANGQENG

Data sets:
Data typeCount
1H chemical shifts202

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1S25p-3C31
2mAb3c32

Entities:

Entity 1, S25p-3C3 20 residues - 1941 Da.

Residue 1-20 represents part (residues 16-35) of the MH2 Gallus gallus MARCKS domain

1   GLULYSPROGLYGLUALAVALALAALASEP
2   PROSERLYSALAASNGLYGLNGLUASNGLY

Entity 2, mAb3c3 - C9 H15 N9 O2 - 281.275 Da.

1   3C3

Samples:

sample_S25p-mAb3c3: MARCKS peptides 1.2 mM; monoclonal antibody 3C3 360 mM; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 13.7 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 13.7 mM; pH: 7.2; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_S25p-mAb3c3isotropicsample_conditions_1
2D 1H-1H NOESYsample_S25p-mAb3c3isotropicsample_conditions_1
2D 1H-1H TOCSYsample_S25p-mAb3c3isotropicsample_conditions_2
2D 1H-1H NOESYsample_S25p-mAb3c3isotropicsample_conditions_2

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 18105