BMRB Entry 18105
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18105
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Citation: Tinoco, Luzineide; Fraga, Jully; AnoBom, Cristiane; Zolessi, Flavio; Obal, Gonzalo; Toledo, Andrea; Pritsch, Otto; Arruti, Cristina. "Structural characterization of a neuroblast-specific phosphorylated region of MARCKS" Biochim. Biophys. Acta 1844, 837-849 (2014).
PubMed: 24590112
Assembly members:
MARCKS_peptides, polymer, 20 residues, 1941 Da.
Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
MARCKS_peptides: EKPGEAVAAXPSKANGQENG
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | S25p | 1 |
Entities:
Entity 1, S25p 20 residues - 1941 Da.
Residue 1-20 represents part (residues 16-35) of the MH2 Gallus gallus MARCKS domain
| 1 | GLU | LYS | PRO | GLY | GLU | ALA | VAL | ALA | ALA | SEP | |
| 2 | PRO | SER | LYS | ALA | ASN | GLY | GLN | GLU | ASN | GLY |
Samples:
sample_S25p: MARCKS peptides 1.2 mM; sodium phosphate 10 mM
sample_conditions_1: ionic strength: 13.7 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K
sample_conditions_2: ionic strength: 13.7 mM; pH: 7.2; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_S25p | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_S25p | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Bruker DRX 600 MHz
- Varian INOVA 600 MHz