BMRB Entry 18103

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18103

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Title:
Backbone resonance assignments for G protein alpha i3 subunit in the GTP-bound state
Deposition date:
2011-11-26
Original release date:
2012-02-01
Authors:
Mase, Yoko; Yokogawa, Mariko; Osawa, Masanori; Shimada, Ichio
Citation:

Citation: Mase, Yoko; Yokogawa, Mariko; Osawa, Masanori; Shimada, Ichio. "Backbone resonance assignments for G protein (i3) subunit in the GTP-bound state."  Biomol. NMR Assignments 6, 217-220 (2012).
PubMed: 22274999

Assembly members:

Assembly members:
G_protein_alphai3_subunit, polymer, 359 residues, Formula weight is not available
deltaN-G_protein_alphai3_subunit, polymer, 329 residues, Formula weight is not available
GSP, non-polymer, 539.246 Da.
MG, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24d(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
G_protein_alphai3_subunit: GPDDHMGCTLSAEDKAAVER SKMIDRNLREDGEKAAKEVK LLLLGAGESGKSTIVKQMKI IHEDGYSEDECKQYKVVVYS NTIQSIIAIIRAMGRLKIDF GEAARADDARQLFVLAGSAE EGVMTPELAGVIKRLWRDGG VQACFSRSREYQLNDSASYY LNDLDRISQSNYIPTQQDVL RTRVKTTGIVETHFTFKDLY FKMFDVGGQRSERKKWIHCF EGVTAIIFCVALSDYDLVLA EDEEMNRMHESMKLFDSICN NKWFTETSIILFLNKKDLFE EKIKRSPLTICYPEYTGSNT YEEAAAYIQCQFEDLNRRKD TKEIYTHFTCATDTKNVQFV FDAVTDVIIKNNLKECGLY
deltaN-G_protein_alphai3_subunit: GPDDHAKEVKLLLLGAGESG KSTIVKQMKIIHEDGYSEDE CKQYKVVVYSNTIQSIIAII RAMGRLKIDFGEAARADDAR QLFVLAGSAEEGVMTPELAG VIKRLWRDGGVQACFSRSRE YQLNDSASYYLNDLDRISQS NYIPTQQDVLRTRVKTTGIV ETHFTFKDLYFKMFDVGGQR SERKKWIHCFEGVTAIIFCV ALSDYDLVLAEDEEMNRMHE SMKLFDSICNNKWFTETSII LFLNKKDLFEEKIKRSPLTI CYPEYTGSNTYEEAAAYIQC QFEDLNRRKDTKEIYTHFTC ATDTKNVQFVFDAVTDVIIK NNLKECGLY

Data sets:
Data typeCount
13C chemical shifts1004
15N chemical shifts337
1H chemical shifts337

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1G_protein_alphai3_subunit1
2deltaN-G_protein_alphai3_subunit2
3GSP3
4magnesium ions4

Entities:

Entity 1, G_protein_alphai3_subunit 359 residues - Formula weight is not available

1   GLYPROASPASPHISMETGLYCYSTHRLEU
2   SERALAGLUASPLYSALAALAVALGLUARG
3   SERLYSMETILEASPARGASNLEUARGGLU
4   ASPGLYGLULYSALAALALYSGLUVALLYS
5   LEULEULEULEUGLYALAGLYGLUSERGLY
6   LYSSERTHRILEVALLYSGLNMETLYSILE
7   ILEHISGLUASPGLYTYRSERGLUASPGLU
8   CYSLYSGLNTYRLYSVALVALVALTYRSER
9   ASNTHRILEGLNSERILEILEALAILEILE
10   ARGALAMETGLYARGLEULYSILEASPPHE
11   GLYGLUALAALAARGALAASPASPALAARG
12   GLNLEUPHEVALLEUALAGLYSERALAGLU
13   GLUGLYVALMETTHRPROGLULEUALAGLY
14   VALILELYSARGLEUTRPARGASPGLYGLY
15   VALGLNALACYSPHESERARGSERARGGLU
16   TYRGLNLEUASNASPSERALASERTYRTYR
17   LEUASNASPLEUASPARGILESERGLNSER
18   ASNTYRILEPROTHRGLNGLNASPVALLEU
19   ARGTHRARGVALLYSTHRTHRGLYILEVAL
20   GLUTHRHISPHETHRPHELYSASPLEUTYR
21   PHELYSMETPHEASPVALGLYGLYGLNARG
22   SERGLUARGLYSLYSTRPILEHISCYSPHE
23   GLUGLYVALTHRALAILEILEPHECYSVAL
24   ALALEUSERASPTYRASPLEUVALLEUALA
25   GLUASPGLUGLUMETASNARGMETHISGLU
26   SERMETLYSLEUPHEASPSERILECYSASN
27   ASNLYSTRPPHETHRGLUTHRSERILEILE
28   LEUPHELEUASNLYSLYSASPLEUPHEGLU
29   GLULYSILELYSARGSERPROLEUTHRILE
30   CYSTYRPROGLUTYRTHRGLYSERASNTHR
31   TYRGLUGLUALAALAALATYRILEGLNCYS
32   GLNPHEGLUASPLEUASNARGARGLYSASP
33   THRLYSGLUILETYRTHRHISPHETHRCYS
34   ALATHRASPTHRLYSASNVALGLNPHEVAL
35   PHEASPALAVALTHRASPVALILEILELYS
36   ASNASNLEULYSGLUCYSGLYLEUTYR

Entity 2, deltaN-G_protein_alphai3_subunit 329 residues - Formula weight is not available

1   GLYPROASPASPHISALALYSGLUVALLYS
2   LEULEULEULEUGLYALAGLYGLUSERGLY
3   LYSSERTHRILEVALLYSGLNMETLYSILE
4   ILEHISGLUASPGLYTYRSERGLUASPGLU
5   CYSLYSGLNTYRLYSVALVALVALTYRSER
6   ASNTHRILEGLNSERILEILEALAILEILE
7   ARGALAMETGLYARGLEULYSILEASPPHE
8   GLYGLUALAALAARGALAASPASPALAARG
9   GLNLEUPHEVALLEUALAGLYSERALAGLU
10   GLUGLYVALMETTHRPROGLULEUALAGLY
11   VALILELYSARGLEUTRPARGASPGLYGLY
12   VALGLNALACYSPHESERARGSERARGGLU
13   TYRGLNLEUASNASPSERALASERTYRTYR
14   LEUASNASPLEUASPARGILESERGLNSER
15   ASNTYRILEPROTHRGLNGLNASPVALLEU
16   ARGTHRARGVALLYSTHRTHRGLYILEVAL
17   GLUTHRHISPHETHRPHELYSASPLEUTYR
18   PHELYSMETPHEASPVALGLYGLYGLNARG
19   SERGLUARGLYSLYSTRPILEHISCYSPHE
20   GLUGLYVALTHRALAILEILEPHECYSVAL
21   ALALEUSERASPTYRASPLEUVALLEUALA
22   GLUASPGLUGLUMETASNARGMETHISGLU
23   SERMETLYSLEUPHEASPSERILECYSASN
24   ASNLYSTRPPHETHRGLUTHRSERILEILE
25   LEUPHELEUASNLYSLYSASPLEUPHEGLU
26   GLULYSILELYSARGSERPROLEUTHRILE
27   CYSTYRPROGLUTYRTHRGLYSERASNTHR
28   TYRGLUGLUALAALAALATYRILEGLNCYS
29   GLNPHEGLUASPLEUASNARGARGLYSASP
30   THRLYSGLUILETYRTHRHISPHETHRCYS
31   ALATHRASPTHRLYSASNVALGLNPHEVAL
32   PHEASPALAVALTHRASPVALILEILELYS
33   ASNASNLEULYSGLUCYSGLYLEUTYR

Entity 3, GSP - C10 H16 N5 O13 P3 S - 539.246 Da.

1   GSP

Entity 4, magnesium ions - Mg - 24.305 Da.

1   MG

Samples:

sample_1: G_protein_alphai3_subunit, [U-13C; U-15N; U-2H], 0.3 mM; HEPES 10 mM; MgCl2 10 mM; GSP 0.8 mM; DSS 1 mM; H2O 90%; D2O 10%

sample_2: deltaN-G_protein_alphai3_subunit, [U-13C; U-15N; 2H], 0.3 mM; HEPES 10 mM; MgCl2 10 mM; GSP 0.8 mM; DSS 1 mM; H2O 90%; D2O 10%

sample_3: deltaN-G_protein_alphai3_subunit, [U-13C; U-15N; 50%-2H], 0.3 mM; HEPES 10 mM; MgCl2 10 mM; GSP 0.3 mM; DSS 1 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSY-HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D 15N-edited NOESY-TROSYsample_1isotropicsample_conditions_1
2D 1H-15N TROSY-HSQCsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(COCA)CBsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
3D 15N-edited NOESY-TROSYsample_2isotropicsample_conditions_1
2D 1H-15N TROSY-HSQCsample_3isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
3D HN(CO)CAsample_3isotropicsample_conditions_1
3D HNCACBsample_3isotropicsample_conditions_1
3D HN(COCA)CBsample_3isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D HN(CA)COsample_3isotropicsample_conditions_1
3D 15N-edited NOESY-TROSYsample_3isotropicsample_conditions_1

Software:

TOPSPIN vv2.1, Bruker Biospin - collection, processing

CARA vv1.8.4.2, Rochus Keller - chemical shift assignment, data analysis, peak picking

Rowland NMR Toolkit, Mobli M, Maciejewski MW, Gryk MR, Hoch JC - processing

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 19015 19015
PDB
DBJ BAA04766 BAB23377 BAC36828 BAE21821 BAE39524 BAA04766 BAB23377 BAC36828 BAE21821 BAE39524
GB AAA35895 AAA35896 AAA35939 AAA40823 AAA41224 AAA35895 AAA35896 AAA35939 AAA40823 AAA41224
REF NP_001029849 NP_001116589 NP_001166426 NP_001230987 NP_001252902 NP_001029849 NP_001116589 NP_001166426 NP_001230987 NP_001252902
SP P08753 P08754 P38403 Q9DC51 P08753 P08754 P38403 Q9DC51
TPG DAA31484 DAA31484
AlphaFold P08753 P08754 P38403 Q9DC51 P08753 P08754 P38403 Q9DC51

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks