BMRB Entry 19015

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19015

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Title:
Backbone resonance assignments for G protein alpha i3 subunit in the GDP-bound state
Deposition date:
2013-02-07
Original release date:
2013-06-28
Authors:
Mase, Yoko; Yokogawa, Mariko; Osawa, Masanori; Shimada, Ichio
Citation:

Citation: Mase, Yoko; Yokogawa, Mariko; Osawa, Masanori; Shimada, Ichio. "Backbone resonance assignments for G protein alpha i3 subunit in the GDP-bound state"  Biomol. NMR Assignments 8, 237-241 (2014).
PubMed: 23771857

Assembly members:

Assembly members:
G_protein_alphai3_subunit, polymer, 359 residues, Formula weight is not available
deltaN-G_protein_alphai3_subunit, polymer, 329 residues, Formula weight is not available
GUANOSINE-5'-DIPHOSPHATE, non-polymer, 443.201 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24d(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
G_protein_alphai3_subunit: GPDDHMGCTLSAEDKAAVER SKMIDRNLREDGEKAAKEVK LLLLGAGESGKSTIVKQMKI IHEDGYSEDECKQYKVVVYS NTIQSIIAIIRAMGRLKIDF GEAARADDARQLFVLAGSAE EGVMTPELAGVIKRLWRDGG VQACFSRSREYQLNDSASYY LNDLDRISQSNYIPTQQDVL RTRVKTTGIVETHFTFKDLY FKMFDVGGQRSERKKWIHCF EGVTAIIFCVALSDYDLVLA EDEEMNRMHESMKLFDSICN NKWFTETSIILFLNKKDLFE EKIKRSPLTICYPEYTGSNT YEEAAAYIQCQFEDLNRRKD TKEIYTHFTCATDTKNVQFV FDAVTDVIIKNNLKECGLY
deltaN-G_protein_alphai3_subunit: GPDDHAKEVKLLLLGAGESG KSTIVKQMKIIHEDGYSEDE CKQYKVVVYSNTIQSIIAII RAMGRLKIDFGEAARADDAR QLFVLAGSAEEGVMTPELAG VIKRLWRDGGVQACFSRSRE YQLNDSASYYLNDLDRISQS NYIPTQQDVLRTRVKTTGIV ETHFTFKDLYFKMFDVGGQR SERKKWIHCFEGVTAIIFCV ALSDYDLVLAEDEEMNRMHE SMKLFDSICNNKWFTETSII LFLNKKDLFEEKIKRSPLTI CYPEYTGSNTYEEAAAYIQC QFEDLNRRKDTKEIYTHFTC ATDTKNVQFVFDAVTDVIIK NNLKECGLY

Data sets:
Data typeCount
13C chemical shifts925
15N chemical shifts301
1H chemical shifts301

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1G protein alphai3 subunit1
2deltaN-G protein alphai3 subunit2
3GDP3

Entities:

Entity 1, G protein alphai3 subunit 359 residues - Formula weight is not available

1   GLYPROASPASPHISMETGLYCYSTHRLEU
2   SERALAGLUASPLYSALAALAVALGLUARG
3   SERLYSMETILEASPARGASNLEUARGGLU
4   ASPGLYGLULYSALAALALYSGLUVALLYS
5   LEULEULEULEUGLYALAGLYGLUSERGLY
6   LYSSERTHRILEVALLYSGLNMETLYSILE
7   ILEHISGLUASPGLYTYRSERGLUASPGLU
8   CYSLYSGLNTYRLYSVALVALVALTYRSER
9   ASNTHRILEGLNSERILEILEALAILEILE
10   ARGALAMETGLYARGLEULYSILEASPPHE
11   GLYGLUALAALAARGALAASPASPALAARG
12   GLNLEUPHEVALLEUALAGLYSERALAGLU
13   GLUGLYVALMETTHRPROGLULEUALAGLY
14   VALILELYSARGLEUTRPARGASPGLYGLY
15   VALGLNALACYSPHESERARGSERARGGLU
16   TYRGLNLEUASNASPSERALASERTYRTYR
17   LEUASNASPLEUASPARGILESERGLNSER
18   ASNTYRILEPROTHRGLNGLNASPVALLEU
19   ARGTHRARGVALLYSTHRTHRGLYILEVAL
20   GLUTHRHISPHETHRPHELYSASPLEUTYR
21   PHELYSMETPHEASPVALGLYGLYGLNARG
22   SERGLUARGLYSLYSTRPILEHISCYSPHE
23   GLUGLYVALTHRALAILEILEPHECYSVAL
24   ALALEUSERASPTYRASPLEUVALLEUALA
25   GLUASPGLUGLUMETASNARGMETHISGLU
26   SERMETLYSLEUPHEASPSERILECYSASN
27   ASNLYSTRPPHETHRGLUTHRSERILEILE
28   LEUPHELEUASNLYSLYSASPLEUPHEGLU
29   GLULYSILELYSARGSERPROLEUTHRILE
30   CYSTYRPROGLUTYRTHRGLYSERASNTHR
31   TYRGLUGLUALAALAALATYRILEGLNCYS
32   GLNPHEGLUASPLEUASNARGARGLYSASP
33   THRLYSGLUILETYRTHRHISPHETHRCYS
34   ALATHRASPTHRLYSASNVALGLNPHEVAL
35   PHEASPALAVALTHRASPVALILEILELYS
36   ASNASNLEULYSGLUCYSGLYLEUTYR

Entity 2, deltaN-G protein alphai3 subunit 329 residues - Formula weight is not available

1   GLYPROASPASPHISALALYSGLUVALLYS
2   LEULEULEULEUGLYALAGLYGLUSERGLY
3   LYSSERTHRILEVALLYSGLNMETLYSILE
4   ILEHISGLUASPGLYTYRSERGLUASPGLU
5   CYSLYSGLNTYRLYSVALVALVALTYRSER
6   ASNTHRILEGLNSERILEILEALAILEILE
7   ARGALAMETGLYARGLEULYSILEASPPHE
8   GLYGLUALAALAARGALAASPASPALAARG
9   GLNLEUPHEVALLEUALAGLYSERALAGLU
10   GLUGLYVALMETTHRPROGLULEUALAGLY
11   VALILELYSARGLEUTRPARGASPGLYGLY
12   VALGLNALACYSPHESERARGSERARGGLU
13   TYRGLNLEUASNASPSERALASERTYRTYR
14   LEUASNASPLEUASPARGILESERGLNSER
15   ASNTYRILEPROTHRGLNGLNASPVALLEU
16   ARGTHRARGVALLYSTHRTHRGLYILEVAL
17   GLUTHRHISPHETHRPHELYSASPLEUTYR
18   PHELYSMETPHEASPVALGLYGLYGLNARG
19   SERGLUARGLYSLYSTRPILEHISCYSPHE
20   GLUGLYVALTHRALAILEILEPHECYSVAL
21   ALALEUSERASPTYRASPLEUVALLEUALA
22   GLUASPGLUGLUMETASNARGMETHISGLU
23   SERMETLYSLEUPHEASPSERILECYSASN
24   ASNLYSTRPPHETHRGLUTHRSERILEILE
25   LEUPHELEUASNLYSLYSASPLEUPHEGLU
26   GLULYSILELYSARGSERPROLEUTHRILE
27   CYSTYRPROGLUTYRTHRGLYSERASNTHR
28   TYRGLUGLUALAALAALATYRILEGLNCYS
29   GLNPHEGLUASPLEUASNARGARGLYSASP
30   THRLYSGLUILETYRTHRHISPHETHRCYS
31   ALATHRASPTHRLYSASNVALGLNPHEVAL
32   PHEASPALAVALTHRASPVALILEILELYS
33   ASNASNLEULYSGLUCYSGLYLEUTYR

Entity 3, GDP - C10 H15 N5 O11 P2 - 443.201 Da.

1   GDP

Samples:

sample_1: G protein alphai3 subunit, [U-13C; U-15N; U-2H], 0.3 mM; HEPES 10 mM; MgCl2 10 mM; GDP 0.8 mM; DSS 1 mM; H2O 90%; D2O 10%; DTT 5 mM

sample_2: deltaN-G protein alphai3 subunit, [U-13C; U-15N; U-2H], 0.3 mM; HEPES 10 mM; MgCl2 10 mM; GDP 0.8 mM; DSS 1 mM; H2O 90%; D2O 10%; DTT 5 mM

sample_3: deltaN-G protein alphai3 subunit, [U-13C; U-15N; 50%-2H], 0.3 mM; HEPES 10 mM; MgCl2 10 mM; GDP 0.8 mM; DSS 1 mM; H2O 90%; D2O 10%; DTT 5 mM

sample_conditions_1: pH: 7.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSY-HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 15N-edited NOESY-TROSYsample_1isotropicsample_conditions_1
2D 1H-15N TROSY-HSQCsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(COCA)CBsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
2D 1H-15N TROSY-HSQCsample_3isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
3D HN(CO)CAsample_3isotropicsample_conditions_1
3D HNCACBsample_3isotropicsample_conditions_1
3D HN(COCA)CBsample_3isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1

Software:

TOPSPIN vv2.1, Bruker Biospin - collection, processing

CARA vv1.8.4.2, Rochus Keller - chemical shift assignment, data analysis, peak picking

Rowland NMR Toolkit, Jeffrey C. Hoch and Alan S. Stern - processing

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

UNP P08754
GB AAM12621.1
AlphaFold Q5TZX1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks