BMRB Entry 18047

Title:
NMR structure of the protein NP_814968.1 from Enterococcus faecalis
Deposition date:
2011-11-08
Original release date:
2011-11-28
Authors:
Susac, Lukas; Serrano, Pedro; Geralt, Michael; Mohanty, Biswarajan; JCSG, JCSG; Wuthrich, Kurt
Citation:

Citation: Susac, Lukas; Serrano, Pedro; Geralt, Michael; Mohanty, Biswarajan; Wuthrich, Kurt. "NMR structure of the protein NP_814968.1 from Enterococcus faecalis"  .

Assembly members:

Assembly members:
NP_814968.1, polymer, 143 residues, 16330.557 Da.

Natural source:

Natural source:   Common Name: Enterococcus faecalis   Taxonomy ID: 1351   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Enterococcus faecalis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSpeedET

Experimental source:

Natural source:   Common Name: Enterococcus faecalis   Taxonomy ID: 1351   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Enterococcus faecalis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSpeedET

Data sets:
Data typeCount
13C chemical shifts484
15N chemical shifts147
1H chemical shifts986

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NP_814968.11

Entities:

Entity 1, NP_814968.1 143 residues - 16330.557 Da.

1   METASPGLUVALLEUALATHRSERILEGLY
2   THRTYRGLUASNARGTHRLEULEUVALPRO
3   ASPGLYARGLEUALALEUTHRALALEUHIS
4   LYSGLYLYSASPTYRGLNGLYLYSPROMET
5   PHETYRVALLEUPHEGLULEUTHRASNTHR
6   THRGLULYSTHRGLNASNILEGLNLEUMET
7   ILEGLNSERPHEMETGLUVALSERGLNTHR
8   VALHISGLYLYSALAGLNASNLEUGLNTYR
9   ALAVALLEUTHRASPSERPROPHEGLNASP
10   LYSLEUASPARGLEUALAASPGLUILEASN
11   PROGLYGLUTHRILEGLNGLYALATYRPRO
12   TYRGLUPHEILEASNGLUASNLYSPROVAL
13   HISPHELYSPHEARGASPARGLEULEUSER
14   LEUASPGLUPROILEALASERGLUGLUILE
15   THRILETHR

Samples:

sample_1: NP_814968.1, [U-95% 13C; U-95% 15N], 1.2 mM; sodium chloride 50 mM; sodium phosphate 20 mM; sodium azide 5 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.083 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
4D APSY HACANHsample_1isotropicsample_conditions_1
5D APSY CBCACONHsample_1isotropicsample_conditions_1
5D APSY HACACONHsample_1isotropicsample_conditions_1

Software:

CYANA, G??ntert P. - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
EMBL CBL31422 CCO72142 CPW23431 CRH64002
GB AAO81038 ADX79912 AEA93699 AFO43932 AHI40381
REF NP_814968 WP_002384665 WP_002395822 WP_002397838 WP_002419155

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks