BMRB Entry 17969

Name: Solution structure and binding studies of the RanBP2-type zinc finger of RBM5
Published: 2012-01-05

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PDB ID: 2lk0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17969
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure and binding studies of the RanBP2-type zinc finger of RBM5

Deposition date:

2011-09-30

Original release date:

2012-01-05

Authors:

Farina, Biancamaria; Pellecchia, Maurizio

Citation:

Citation: Farina, Biancamaria; Fattorusso, Roberto; Pellecchia, Maurizio. "Targeting Zinc Finger Domains with Small Molecules: Solution Structure and Binding Studies of the RanBP2-Type Zinc Finger of RBM5" ChemBioChem 12, 2837-2845 (2011).
PubMed: 22162216

Assembly members:

Assembly members:
RBM5-ZF1, polymer, 32 residues, 3734.461 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Homo sapiens Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RBM5-ZF1: XKFEDWLCNKCCLNNFRKRL KCFRCGADKFDX

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	214

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RBM5-ZF1	1
2	ZINC ION	2

Entities:

Entity 1, RBM5-ZF1 32 residues - 3734.461 Da.

1

ACE

LYS

PHE

GLU

ASP

TRP

LEU

CYS

ASN

LYS

2

CYS

LEU

ASN

PHE

ARG

LYS

ARG

LEU

3

LYS

CYS

PHE

ARG

CYS

GLY

ALA

ASP

LYS

PHE

4

ASP

NH2

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: RBM5-ZF1 100 uM; ZINC ION 150 uM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0 M; pH: 5.7; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN vv2.1, Bruker Biospin - collection, data analysis

CARA vv1.5,v1.8, Keller and Wuthrich - chemical shift assignment, peak picking

CYANA vv2.1, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

SYBYL, Tripos - Energy Minimization, refinement

NMR spectrometers:

Bruker Avance 700 MHz

BMRB	17387 17970
PDB	2LK0 2LK1
DBJ	BAE22042 BAE27501 BAE87995 BAG36742 BAG59728
EMBL	CAC69136
GB	AAA99715 AAB42216 AAD04159 AAF02422 AAH19766
REF	NP_001039839 NP_001094018 NP_001248103 NP_005769 NP_683732
SP	B2GV05 P52756 Q1RMU5 Q91YE7
TPG	DAA16943
AlphaFold	B2GV05 P52756 Q1RMU5 Q91YE7