BMRB Entry 17938

Title:
Solution Chemical Shifts Assignments of 1H, 13C, and 15N for CAP-Gly   PubMed: 19580321
Deposition date:
2011-09-14
Original release date:
2011-10-28
Authors:
Yan, Si
Citation:

Citation: Sun, Shangjin; Siglin, Amanda; Williams, John; Polenova, Tatyana. "Solid-State and Solution NMR Studies of the CAP-Gly Domain of Mammalian Dynactin and Its Interaction with Microtubules"  J. Am. Chem. Soc. 131, 10113-10126 (2009).

Assembly members:

Assembly members:
CAP-Gly, polymer, 88 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b-His6-SMT3

Experimental source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b-His6-SMT3

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts247
15N chemical shifts86
1H chemical shifts300

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CAP-Gly1

Entities:

Entity 1, CAP-Gly 88 residues - Formula weight is not available

1   THRGLUALASERALAARGPROLEUARGVAL
2   GLYSERARGVALGLUVALILEGLYLYSGLY
3   HISARGGLYTHRVALALATYRVALGLYALA
4   THRLEUPHEALATHRGLYLYSTRPVALGLY
5   VALILELEUASPGLUALALYSGLYLYSASN
6   ASPGLYTHRVALGLNGLYARGLYSTYRPHE
7   THRCYSASPGLUGLYHISGLYILEPHEVAL
8   ARGGLNSERGLNILEGLNVALPHEGLUASP
9   GLYALAASPTHRTHRSERPROGLU

Samples:

sample_1: CAP-Gly, [U-100% 13C; U-100% 15N], 0.96 mM

sample_conditions_1: ionic strength: 90 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 17937 19025 19031 25005
PDB
DBJ BAE34241 BAE37079 BAE42418 BAE42912 BAE87998
EMBL CAA44091 CAA67333 CAE45882 CAH10572 CAH10575
GB AAB57773 AAD03694 AAD55811 AAH66061 AAI42510
REF NP_001092404 NP_001127253 NP_001128512 NP_001177765 NP_001177766
SP O08788 P28023 Q14203
TPG DAA24857
AlphaFold Q14203

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts