BMRB Entry 17937

Title:
Solid-state Chemical Shift Assignments for CAP-Gly Domain of Mammalian Dynactin   PubMed: 19580321
Deposition date:
2011-09-14
Original release date:
2011-10-28
Authors:
Yan, Si
Citation:

Citation: Sun, Shangjin; Siglin, Amanda; Williams, John; Polenova, Tatyana. "Solid-State and Solution NMR Studies of the CAP-Gly Domain of Mammalian Dynactin and Its Interaction with Microtubules"  J. Am. Chem. Soc. 131, 10113-10126 (2009).

Assembly members:

Assembly members:
CAP-Gly, polymer, 88 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b-His6-SMT3

Experimental source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b-His6-SMT3

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts327
15N chemical shifts85

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CAP-Gly1

Entities:

Entity 1, CAP-Gly 88 residues - Formula weight is not available

1   THRGLUALASERALAARGPROLEUARGVAL
2   GLYSERARGVALGLUVALILEGLYLYSGLY
3   HISARGGLYTHRVALALATYRVALGLYALA
4   THRLEUPHEALATHRGLYLYSTRPVALGLY
5   VALILELEUASPGLUALALYSGLYLYSASN
6   ASPGLYTHRVALGLNGLYARGLYSTYRPHE
7   THRCYSASPGLUGLYHISGLYILEPHEVAL
8   ARGGLNSERGLNILEGLNVALPHEGLUASP
9   GLYALAASPTHRTHRSERPROGLU

Samples:

sample_1: CAP-Gly, [U-100% 13C; U-100% 15N], 14 ± 0.5 mg

sample_conditions_1: ionic strength: 30 mM; pH: 6.0; pressure: 1 atm; temperature: 256.3 K

sample_conditions_2: ionic strength: 30 mM; pH: 6.0; pressure: 1 atm; temperature: 258 K

Experiments:

NameSampleSample stateSample conditions
DARRsample_1solidsample_conditions_2
NCAsample_1solidsample_conditions_1
NCOsample_1solidsample_conditions_1
NCACBsample_1solidsample_conditions_1
NCACXsample_1solidsample_conditions_1
CTUC-COCAsample_1solidsample_conditions_1
CTUC-CACBsample_1solidsample_conditions_1
CTUC-NCOCAsample_1solidsample_conditions_1
NCOCXsample_1solidsample_conditions_1
DARRsample_1solidsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

Rowland NMR Toolkit, (RNMRTK)-Hoch, Stern, Li, Mobli, Maciejewski, Gryk - processing

NMR spectrometers:

  • Varian InfinityPlus 600 MHz
  • Varian INOVA 900 MHz

Related Database Links:

BMRB 17938 19025 19031 25005
PDB
DBJ BAE34241 BAE37079 BAE42418 BAE42912 BAE87998
EMBL CAA44091 CAA67333 CAE45882 CAH10572 CAH10575
GB AAB57773 AAD03694 AAD55811 AAH66061 AAI42510
REF NP_001092404 NP_001127253 NP_001128512 NP_001177765 NP_001177766
SP O08788 P28023 Q14203
TPG DAA24857
AlphaFold O08788