BMRB Entry 17925
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17925
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Citation: Sheftic, Sarah; Snell, Jessica; Jha, Suman; Alexandrescu, Andrei. "Inhibition of semen-derived enhancer of virus infection (SEVI) fibrillogenesis by zinc and copper." Eur. Biophys. J. 41, 695-704 (2012).
PubMed: 22907203
Assembly members:
SEVI, polymer, 39 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
SEVI: GIHKQKEKSRLQGGVLVNEI
LNHMKRATQIPSYKKLIMY
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 39 |
| 1H chemical shifts | 143 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SEVI | 1 |
| 2 | Zn | 2 |
Entities:
Entity 1, SEVI 39 residues - Formula weight is not available
| 1 | GLY | ILE | HIS | LYS | GLN | LYS | GLU | LYS | SER | ARG | ||||
| 2 | LEU | GLN | GLY | GLY | VAL | LEU | VAL | ASN | GLU | ILE | ||||
| 3 | LEU | ASN | HIS | MET | LYS | ARG | ALA | THR | GLN | ILE | ||||
| 4 | PRO | SER | TYR | LYS | LYS | LEU | ILE | MET | TYR |
Entity 2, Zn - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: SEVI 3.0 mM; Zn 4.0 mM
sample_conditions_1: ionic strength: 0 M; pH: 5.6; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ, Varian - data analysis
FELIX, Accelrys Software Inc. - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Varian INOVA 600 MHz