BMRB Entry 17193
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17193
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Nanga, Ravi Prakash Reddy; Brender, Jeffrey Robert; Vivekanandan, Subramanian; Popovych, Nataliya; Ramamoorthy, Ayyalusamy. "NMR structure in a membrane environment reveals putative amyloidogenic regions of the SEVI precursor peptide PAP(248-286)." J. Am. Chem. Soc. 131, 17972-17979 (2009).
PubMed: 19995078
Assembly members:
PAP248-286, polymer, 39 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
PAP248-286: GIHKQKEKSRLQGGVLVNEI
LNHMKRATQIPSYKKLIMY
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 253 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PAP248-286 | 1 |
Entities:
Entity 1, PAP248-286 39 residues - Formula weight is not available
| 1 | GLY | ILE | HIS | LYS | GLN | LYS | GLU | LYS | SER | ARG | ||||
| 2 | LEU | GLN | GLY | GLY | VAL | LEU | VAL | ASN | GLU | ILE | ||||
| 3 | LEU | ASN | HIS | MET | LYS | ARG | ALA | THR | GLN | ILE | ||||
| 4 | PRO | SER | TYR | LYS | LYS | LEU | ILE | MET | TYR |
Samples:
sample_1: PAP248-286 2.5 mM; sodium chloride 120 mM; sodium phosphate 20 mM; SDS, [U-99% 2H], 200 mM; D2O, [U-99% 2H], 10%; H2O 10%
sample_conditions_1: ionic strength: 20 mM; pH: 7.3; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution
TOPSPIN v2.16, Bruker Biospin - collection, processing
SPARKY, Goddard - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker Avance 600 MHz