BMRB Entry 17761

Name: Backbone 1H, 13C, 15N and 13CB assignment of N-terminal Domain of Ribosomal Protein L9 (NTL9) Denatured State in 8.3M Urea
Published: 2012-09-20

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17761

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, 15N and 13CB assignment of N-terminal Domain of Ribosomal Protein L9 (NTL9) Denatured State in 8.3M Urea

Deposition date:

2011-07-05

Original release date:

2012-09-20

Authors:

Meng, Wenli; Raleigh, Daniel

Citation:

Citation: Meng, Wenli; Raleigh, Daniel. "Analysis of electrostatic interactions in the denatured state ensemble of the N-terminal domain of L9 under native conditions." Proteins 79, 3500-3510 (2011).
PubMed: 21915914

Assembly members:

Assembly members:
N-terminal Domain of the Ribosomal Protein L9, polymer, 56 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Geobacillus stearothermophilus Taxonomy ID: 1422 Superkingdom: Bacteria Kingdom: not available Genus/species: Geobacillus stearothermophilus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
N-terminal Domain of the Ribosomal Protein L9: MKVIFLKDVKGKGKKGEIKN VADGYANNFLFKQGLAIEAT PANLKALEAQKQKEQR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	161
15N chemical shifts	54
1H chemical shifts	108

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	N-terminal Domain of the Ribosomal Protein L9	1

Entities:

Entity 1, N-terminal Domain of the Ribosomal Protein L9 56 residues - Formula weight is not available

1

MET

LYS

VAL

ILE

PHE

LEU

LYS

ASP

VAL

LYS

2

GLY

LYS

GLY

LYS

GLY

GLU

ILE

LYS

ASN

3

VAL

ALA

ASP

GLY

TYR

ALA

ASN

PHE

LEU

4

PHE

LYS

GLN

GLY

LEU

ALA

ILE

GLU

ALA

THR

5

PRO

ALA

ASN

LEU

LYS

ALA

LEU

GLU

ALA

GLN

6

LYS

GLN

LYS

GLU

GLN

ARG

Samples:

sample_1: sodium chloride 100 mM; sodium acetate 20 mM; urea 8.3 M; DSS 0.4 mM; N-terminal Domain of the Ribosomal Protein L9, [U-100% 13C; U-100% 15N], 1 mM; H2O 90%; D2O 10%

sample_2: sodium chloride 100 mM; sodium acetate 20 mM; urea 8.3 M; DSS 0.4 mM; N-terminal Domain of the Ribosomal Protein L9, [U-100% 15N], 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.12 M; pH: 5.5; pressure: 1 atm; temperature: 285 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_2	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker Avance 500 MHz

BMRB	17460 17506 4551
PDB	1CQU 1DIV 1GIY 1YL3 2B66 2B9N 2B9P 2HBA 2HBB 2HVF 487D
EMBL	CAA43972
GB	AAA22701 ABO68755 AGT33858 AKM20686 AKU26730
PRF	0701226A 1714237A
REF	WP_008880809 WP_011888453 WP_020961638 WP_033008865 WP_050368025
SP	A4ITV1 P02417
AlphaFold	A4ITV1 P02417