BMRB Entry 17639

Name: NMR Structure of the Complete Internal Fusion Loop from Ebolavirus GP2 at pH 7.0
Published: 2011-08-03

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PDB ID: 2lcz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17639
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Structure of the Complete Internal Fusion Loop from Ebolavirus GP2 at pH 7.0

Deposition date:

2011-05-12

Original release date:

2011-08-03

Authors:

Gregory, S.; Harada, E.; Liang, B.; Tamm, L.

Citation:

Citation: Gregory, Sonia; Harada, Erisa; Liang, Binyong; Delos, Sue; White, Judith; Tamm, Lukas. "Structure and function of the complete internal fusion loop from Ebolavirus glycoprotein 2." Proc. Natl. Acad. Sci. U. S. A. ., .-. (2011).
PubMed: 21690393

Assembly members:

Assembly members:
Ebolavirus_Fusion_Loop_pH_7.0, polymer, 54 residues, 5947.741 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET41

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ebolavirus_Fusion_Loop_pH_7.0: AQPKCNPNLHYWTTQDEGAA IGLAWIPYFGPAAEGIYIEG LMHNQDGLICGLRQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	228
15N chemical shifts	58
1H chemical shifts	356

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ebolavirus Fusion Loop pH 7.0	1

Entities:

Entity 1, Ebolavirus Fusion Loop pH 7.0 54 residues - 5947.741 Da.

1

ALA

GLN

PRO

LYS

CYS

ASN

PRO

ASN

LEU

HIS

2

TYR

TRP

THR

GLN

ASP

GLU

GLY

ALA

3

ILE

GLY

LEU

ALA

TRP

ILE

PRO

TYR

PHE

GLY

4

PRO

ALA

GLU

GLY

ILE

TYR

ILE

GLU

GLY

5

LEU

MET

HIS

ASN

GLN

ASP

GLY

LEU

ILE

CYS

6

GLY

LEU

ARG

GLN

Samples:

sample_1: Ebolavirus Fusion Loop pH 7.0, [U-13C; U-15N], 0.5 – 1 mM; H2O 90%; D2O 10%

sample_2: Ebolavirus Fusion Loop pH 7.0, [U-15N], 0.5 – 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

NMR spectrometers:

Bruker DRX 800 MHz
Bruker DRX 600 MHz
Varian NMRS 600 MHz

BMRB	17638 19383
PDB	2LCY 2LCZ 2MB1 3CSY
GB	AAA96744 AAB37095 AAB81004 AAC54887 AAC57989
REF	NP_066246
SP	O11457 P87666 Q05320
AlphaFold	O11457 P87666 Q05320