BMRB Entry 17483
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17483
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NMR-STAR v3 text file.
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Citation: Singarapu, Kiran; Tonelli, Marco; Chow, Darius; Frederick, Ronnie; Westler, William; Markley, John. "Structural characterization of Hsp12, the heat shock protein from Saccharomyces cerevisiae, in aqueous solution where it is intrinsically disordered and in detergent micelles where it is locally -helical." J. Biol. Chem. 286, 43447-43453 (2011).
PubMed: 21998307
Assembly members:
hsp12, polymer, 109 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pvp68k
Entity Sequences (FASTA):
hsp12: MSDAGRKGFGEKASEALKPD
SQKSYAEQGKEYITDKADKV
AGKVQPEDNKGVFQGVHDSA
EKGKDNAEGQGESLADQARD
YMGAAKSKLNDAVEYVSGRV
HGEEDPTKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 293 |
| 15N chemical shifts | 99 |
| 1H chemical shifts | 387 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hsp12 | 1 |
Entities:
Entity 1, hsp12 109 residues - Formula weight is not available
| 1 | MET | SER | ASP | ALA | GLY | ARG | LYS | GLY | PHE | GLY | ||||
| 2 | GLU | LYS | ALA | SER | GLU | ALA | LEU | LYS | PRO | ASP | ||||
| 3 | SER | GLN | LYS | SER | TYR | ALA | GLU | GLN | GLY | LYS | ||||
| 4 | GLU | TYR | ILE | THR | ASP | LYS | ALA | ASP | LYS | VAL | ||||
| 5 | ALA | GLY | LYS | VAL | GLN | PRO | GLU | ASP | ASN | LYS | ||||
| 6 | GLY | VAL | PHE | GLN | GLY | VAL | HIS | ASP | SER | ALA | ||||
| 7 | GLU | LYS | GLY | LYS | ASP | ASN | ALA | GLU | GLY | GLN | ||||
| 8 | GLY | GLU | SER | LEU | ALA | ASP | GLN | ALA | ARG | ASP | ||||
| 9 | TYR | MET | GLY | ALA | ALA | LYS | SER | LYS | LEU | ASN | ||||
| 10 | ASP | ALA | VAL | GLU | TYR | VAL | SER | GLY | ARG | VAL | ||||
| 11 | HIS | GLY | GLU | GLU | ASP | PRO | THR | LYS | LYS |
Samples:
sample_1: hsp12, [U-100% 15N], 0.2 mM; hsp12, [U-100% 13C; U-100% 15N], 1.0 mM
sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ, Varian - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
XEASY, Bartels et al. - data analysis
PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment
TALOS, Cornilescu, Delaglio and Bax - data analysis
NMR spectrometers:
- Varian INOVA 900 MHz
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 17482 17948 18523 |
| PDB | |
| DBJ | BAA09224 BAA14033 GAA23069 |
| EMBL | CAA39306 CAA86349 CAY79436 |
| GB | AAA34647 AAL06077 AAS56790 AHY75775 AJP38487 |
| REF | NP_116640 |
| SP | P22943 |
| TPG | DAA12425 |
| AlphaFold | P22943 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks