BMRB Entry 18523
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18523
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Herbert, Andrew; Riesen, Michele; Bloxam, Leanne; Kosmidou, Effie; Wareing, Brian; Johnson, James; Phelan, Marie; Pennington, Stephen; Lian, Lu-Yun; Morgan, Alan. "NMR structure of hsp12, a protein induced by and required for dietary restriction-induced lifespan extension in yeast" PLOS One 7, e41975-e41975 (2012).
PubMed: 22848679
Assembly members:
HSP12_SDS, polymer, 109 residues, 11690.6574 Da.
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pE-SUMO-Pro-Kan
Entity Sequences (FASTA):
HSP12_SDS: MSDAGRKGFGEKASEALKPD
SQKSYAEQGKEYITDKADKV
AGKVQPEDNKGVFQGVHDSA
EKGKDNAEGQGESLADQARD
YMGAAKSKLNDAVEYVSGRV
HGEEDPTKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 425 |
| 15N chemical shifts | 111 |
| 1H chemical shifts | 683 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HSP12 SDS | 1 |
Entities:
Entity 1, HSP12 SDS 109 residues - 11690.6574 Da.
| 1 | MET | SER | ASP | ALA | GLY | ARG | LYS | GLY | PHE | GLY | ||||
| 2 | GLU | LYS | ALA | SER | GLU | ALA | LEU | LYS | PRO | ASP | ||||
| 3 | SER | GLN | LYS | SER | TYR | ALA | GLU | GLN | GLY | LYS | ||||
| 4 | GLU | TYR | ILE | THR | ASP | LYS | ALA | ASP | LYS | VAL | ||||
| 5 | ALA | GLY | LYS | VAL | GLN | PRO | GLU | ASP | ASN | LYS | ||||
| 6 | GLY | VAL | PHE | GLN | GLY | VAL | HIS | ASP | SER | ALA | ||||
| 7 | GLU | LYS | GLY | LYS | ASP | ASN | ALA | GLU | GLY | GLN | ||||
| 8 | GLY | GLU | SER | LEU | ALA | ASP | GLN | ALA | ARG | ASP | ||||
| 9 | TYR | MET | GLY | ALA | ALA | LYS | SER | LYS | LEU | ASN | ||||
| 10 | ASP | ALA | VAL | GLU | TYR | VAL | SER | GLY | ARG | VAL | ||||
| 11 | HIS | GLY | GLU | GLU | ASP | PRO | THR | LYS | LYS |
Samples:
13C_15NH_SP12: HSP12_SDS, [U-13C; U-15N], 0.45 mM; SDS 100.00 mM; NaCl 40.00 mM; Potassium Phosphate 20.00 mM
15N_HSP12: SDS 100.00 mM; NaCl 40.00 mM; Potassium Phosphate 20.00 mM; HSP12_SDS, [U-15N], 0.45 mM
SDS: pH: 6.500; pressure: 1.000 atm; temperature: 318.000 K
CondSet1: temperature: 298.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC/HMQC | 13C_15NH_SP12 | isotropic | SDS |
| 3D HNCO | 13C_15NH_SP12 | isotropic | SDS |
| hncaco (H[N[ca[CO]]]) | 13C_15NH_SP12 | isotropic | SDS |
| 3D CBCA(CO)NH | 13C_15NH_SP12 | isotropic | SDS |
| 3D HNCACB | 13C_15NH_SP12 | isotropic | SDS |
| 2D 1H-13C HSQC/HMQC | 13C_15NH_SP12 | isotropic | SDS |
| 3D HNCA | 13C_15NH_SP12 | isotropic | SDS |
| 3D HBHA(CO)NH | 13C_15NH_SP12 | isotropic | SDS |
| 3D 1H-15N NOESY | 15N_HSP12 | isotropic | SDS |
| hbhanh (H{ca|cca}NH) | 13C_15NH_SP12 | isotropic | SDS |
| 3D HCCH-TOCSY | 13C_15NH_SP12 | isotropic | SDS |
| 3D 1H-13C NOESY | 13C_15NH_SP12 | isotropic | SDS |
| c-trosy-arom (H[C[caro]]) | 13C_15NH_SP12 | isotropic | SDS |
| hncbcgcdhd (hbCBcgcdHD) | 13C_15NH_SP12 | isotropic | SDS |
| CBCACONH | 15N_HSP12 | solution | CondSet1 |
| HBHACONH | 15N_HSP12 | solution | CondSet1 |
Software:
AutoDep v4.3, PDBe - collection
CYANA v2.1, GUENTERT - Structure Calculation/refinement
ANALYSIS v2.1, CCPN - NMR Resonance Assignment
DANGLE v1.1, Ming-Sin Cheung et al. - Dihedral restraint calculation
TOPSPIN v2.1, Bruker - Data Acquisition, Spectrum Processing
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks