BMRB Entry 18523

Name: NMR structure of Hsp12, a protein induced by and required for dietary restriction-induced lifespan extension in yeast.
Published: 2012-08-06

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PDB ID: 4axp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18523
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of Hsp12, a protein induced by and required for dietary restriction-induced lifespan extension in yeast.

Deposition date:

2012-06-14

Original release date:

2012-08-06

Authors:

Herbert, Andrew; Lian, Lu-Yun; Morgan, Alan; Riesen, Michele; Bloxam, Leanne; Kosmidou, Effie; Wareing, Brian; Phelan, J.; Pennington, Stephen

Citation:

Citation: Herbert, Andrew; Riesen, Michele; Bloxam, Leanne; Kosmidou, Effie; Wareing, Brian; Johnson, James; Phelan, Marie; Pennington, Stephen; Lian, Lu-Yun; Morgan, Alan. "NMR structure of hsp12, a protein induced by and required for dietary restriction-induced lifespan extension in yeast" PLOS One 7, e41975-e41975 (2012).
PubMed: 22848679

Assembly members:

Assembly members:
HSP12_SDS, polymer, 109 residues, 11690.6574 Da.

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pE-SUMO-Pro-Kan

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HSP12_SDS: MSDAGRKGFGEKASEALKPD SQKSYAEQGKEYITDKADKV AGKVQPEDNKGVFQGVHDSA EKGKDNAEGQGESLADQARD YMGAAKSKLNDAVEYVSGRV HGEEDPTKK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	425
15N chemical shifts	111
1H chemical shifts	683

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HSP12 SDS	1

Entities:

Entity 1, HSP12 SDS 109 residues - 11690.6574 Da.

1

MET

SER

ASP

ALA

GLY

ARG

LYS

GLY

PHE

GLY

2

GLU

LYS

ALA

SER

GLU

ALA

LEU

LYS

PRO

ASP

3

SER

GLN

LYS

SER

TYR

ALA

GLU

GLN

GLY

LYS

4

GLU

TYR

ILE

THR

ASP

LYS

ALA

ASP

LYS

VAL

5

ALA

GLY

LYS

VAL

GLN

PRO

GLU

ASP

ASN

LYS

6

GLY

VAL

PHE

GLN

GLY

VAL

HIS

ASP

SER

ALA

7

GLU

LYS

GLY

LYS

ASP

ASN

ALA

GLU

GLY

GLN

8

GLY

GLU

SER

LEU

ALA

ASP

GLN

ALA

ARG

ASP

9

TYR

MET

GLY

ALA

LYS

SER

LYS

LEU

ASN

10

ASP

ALA

VAL

GLU

TYR

VAL

SER

GLY

ARG

VAL

11

HIS

GLY

GLU

ASP

PRO

THR

LYS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC/HMQC	13C_15NH_SP12	isotropic	SDS
3D HNCO	13C_15NH_SP12	isotropic	SDS
hncaco (H[N[ca[CO]]])	13C_15NH_SP12	isotropic	SDS
3D CBCA(CO)NH	13C_15NH_SP12	isotropic	SDS
3D HNCACB	13C_15NH_SP12	isotropic	SDS
2D 1H-13C HSQC/HMQC	13C_15NH_SP12	isotropic	SDS
3D HNCA	13C_15NH_SP12	isotropic	SDS
3D HBHA(CO)NH	13C_15NH_SP12	isotropic	SDS
3D 1H-15N NOESY	15N_HSP12	isotropic	SDS
hbhanh (H{ca\|cca}NH)	13C_15NH_SP12	isotropic	SDS
3D HCCH-TOCSY	13C_15NH_SP12	isotropic	SDS
3D 1H-13C NOESY	13C_15NH_SP12	isotropic	SDS
c-trosy-arom (H[C[caro]])	13C_15NH_SP12	isotropic	SDS
hncbcgcdhd (hbCBcgcdHD)	13C_15NH_SP12	isotropic	SDS
CBCACONH	15N_HSP12	solution	CondSet1
HBHACONH	15N_HSP12	solution	CondSet1

UNP	HSP12_YEAST
AlphaFold	Q8X145

BMRB Entry 18523

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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