BMRB Entry 17464
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17464
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Citation: Lozano, Rosa; Jimenez, M.; Santoro, Jorge; Rico, Manuel; Gimenez-Gallego, Guillermo. "Solution Structure of Acidic Fibroblast Growth Factor Bound to 1,3,6-Naphthalenetrisulfonate: A Minimal Model for the Anti-tumoral Action of Suramins and Suradistas" J. Mol. Biol. 281, 899-915 (1998).
Assembly members:
FGF, polymer, 132 residues, 365.336 Da.
NTS, non-polymer, 365.336 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMG47
- chemical_rates
| Data type | Count |
| kinetic rates | 1 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FGF | 1 |
| 2 | NTS | 2 |
Entities:
Entity 1, FGF 132 residues - 365.336 Da.
| 1 | LYS | LYS | PRO | LYS | LEU | LEU | TYR | CYS | SER | ASN | ||||
| 2 | GLY | GLY | HIS | PHE | LEU | ARG | ILE | LEU | PRO | ASP | ||||
| 3 | GLY | THR | VAL | ASP | GLY | THR | ARG | ASP | ARG | SER | ||||
| 4 | ASP | GLN | HIS | ILE | GLN | LEU | GLN | LEU | SER | ALA | ||||
| 5 | GLU | SER | VAL | GLY | GLU | VAL | TYR | ILE | LYS | SER | ||||
| 6 | THR | GLU | THR | GLY | GLN | TYR | LEU | ALA | MET | ASP | ||||
| 7 | THR | ASP | GLY | LEU | LEU | TYR | GLY | SER | GLN | THR | ||||
| 8 | PRO | ASN | GLU | GLU | CYS | LEU | PHE | LEU | GLU | ARG | ||||
| 9 | LEU | GLU | GLU | ASN | HIS | TYR | ASN | THR | TYR | ILE | ||||
| 10 | SER | LYS | LYS | HIS | ALA | GLU | LYS | ASN | TRP | PHE | ||||
| 11 | VAL | GLY | LEU | LYS | LYS | ASN | GLY | SER | CYS | LYS | ||||
| 12 | ARG | GLY | PRO | ARG | THR | HIS | TYR | GLY | GLN | LYS | ||||
| 13 | ALA | ILE | LEU | PHE | LEU | PRO | LEU | PRO | VAL | SER | ||||
| 14 | SER | ASP |
Entity 2, NTS - C10 H5 O9 S3 - 365.336 Da.
| 1 | NTS |
Samples:
sample_1: FGF 22 mg/ml; beta mercaptoethanol 1 mM; sodium chloride 150 mM; NTS bound to FGF mM; D2O 100%
sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
LSQKAB, (Collaborative Computational Project, Number 4, 1994). - data analysis
NMR spectrometers:
- Bruker AMX 600 MHz
Related Database Links:
| BMRB | 15783 15960 16493 16494 16502 17674 |
| PDB | |
| DBJ | BAC29035 BAF82451 BAG35227 BAI46827 |
| EMBL | CAA32448 CAA36206 CAA42869 CAA46661 CAI29610 |
| GB | AAA37618 AAA52446 AAA52638 AAA79245 AAB29057 |
| PRF | 1605206A |
| REF | NP_000791 NP_001127073 NP_001138364 NP_001138406 NP_001138407 |
| SP | P05230 P20002 P34004 P61148 P61149 |
| AlphaFold | P05230 P20002 P34004 P61148 P61149 |