BMRB Entry 15960

Title:
Solution structure of human acidic fibroblast growth factor in complex with anti-angiogenic drug inositol hexaphosphate (IP6)
Deposition date:
2008-09-22
Original release date:
2011-06-01
Authors:
Kumar, Sriramoju; Mohan, Sepuru; Chin, Yu
Citation:

Citation: Kumar, Sriramoju; Wang, Han-Min; Mohan, Sepuru; Chou, Ruey-Hwang; Yu, Chin. "Molecular level interaction of the human acidic fibroblast growth factor with the antiangiogenic agent, inositol hexaphosphate ."  Biochemistry 49, 10756-10764 (2010).
PubMed: 21077672

Assembly members:

Assembly members:
FGF-1, polymer, 133 residues, 15118.163 Da.
IHP, non-polymer, 660.035 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET(20)

Data sets:
Data typeCount
13C chemical shifts357
15N chemical shifts130
1H chemical shifts676

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FGF-11
2INOSITOL HEXAKISPHOSPHATE2

Entities:

Entity 1, FGF-1 133 residues - 15118.163 Da.

1   TYRLYSLYSPROLYSLEULEUTYRCYSSER
2   ASNGLYGLYHISPHELEUARGILELEUPRO
3   ASPGLYTHRVALASPGLYTHRARGASPARG
4   SERASPGLNHISILEGLNLEUGLNLEUSER
5   ALAGLUSERVALGLYGLUVALTYRILELYS
6   SERTHRGLUTHRGLYGLNTYRLEUALAMET
7   ASPTHRASPGLYLEULEUTYRGLYSERGLN
8   THRPROASNGLUGLUCYSLEUPHELEUGLU
9   ARGLEUGLUGLUASNHISTYRASNTHRTYR
10   ILESERLYSLYSHISALAGLULYSASNTRP
11   PHEVALGLYLEULYSLYSASNGLYSERCYS
12   LYSARGGLYPROARGTHRHISTYRGLYGLN
13   LYSALAILELEUPHELEUPROLEUPROVAL
14   SERSERASP

Entity 2, INOSITOL HEXAKISPHOSPHATE - C6 H18 O24 P6 - 660.035 Da.

1   IHP

Samples:

sample_1: FGF-1, [U-100% 13C; U-100% 15N], 1.5 mM; INOSITOL HEXAKISPHOSPHATE 1.5 mM; ammonium sulfate 100 mM; sodium phosphate 25 mM; H20 90%; D20 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

ARIA v1.1, Dr. Michael Nilges, Institut Pasteur - Automated NOE assignment, NMR structure calculation

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker DRX 600 MHz

Related Database Links:

BMRB 15783 16493 16494 16502 17464 17674
PDB
DBJ BAC29035 BAF82451 BAG35227 BAI46827
EMBL CAA32448 CAA36206 CAA42869 CAA46661 CAI29610
GB AAA37618 AAA52446 AAA52638 AAA79245 AAB29057
PRF 1605206A
REF NP_000791 NP_001127073 NP_001138364 NP_001138406 NP_001138407
SP P05230 P20002 P34004 P61148 P61149
AlphaFold P05230 P20002 P34004 P61148 P61149

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks