BMRB Entry 17413
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17413
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Citation: Kleckner, Ian; Gollnick, Paul; Foster, Mark. "Mechanisms of allosteric gene regulation by NMR quantification of microsecond-millisecond protein dynamics" J. Mol. Biol. 415, 372-381 (2012).
PubMed: 22115774
Assembly members:
TRAP, polymer, 74 residues, Formula weight is not available
TRP, non-polymer, 204.225 Da.
Natural source: Common Name: Bacillus stearothermophilus Taxonomy ID: 1422 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus stearothermophilus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32
Entity Sequences (FASTA):
TRAP: MYTNSDFVVIKALEDGVNVI
GLTRGIDTRFHHSEKLDKGE
VLIAQFTEHTSAIKVRGKAY
IQTRHGVIESEGKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 29 |
| 1H chemical shifts | 87 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TRAP, chain 1 | 1 |
| 2 | TRAP, chain 2 | 1 |
| 3 | TRAP, chain 3 | 1 |
| 4 | TRAP, chain 4 | 1 |
| 5 | TRAP, chain 5 | 1 |
| 6 | TRAP, chain 6 | 1 |
| 7 | TRAP, chain 7 | 1 |
| 8 | TRAP, chain 8 | 1 |
| 9 | TRAP, chain 9 | 1 |
| 10 | TRAP, chain 10 | 1 |
| 11 | TRAP, chain 11 | 1 |
| 12 | L-tryptophan | 2 |
Entities:
Entity 1, TRAP, chain 1 74 residues - Formula weight is not available
| 1 | MET | TYR | THR | ASN | SER | ASP | PHE | VAL | VAL | ILE | ||||
| 2 | LYS | ALA | LEU | GLU | ASP | GLY | VAL | ASN | VAL | ILE | ||||
| 3 | GLY | LEU | THR | ARG | GLY | ILE | ASP | THR | ARG | PHE | ||||
| 4 | HIS | HIS | SER | GLU | LYS | LEU | ASP | LYS | GLY | GLU | ||||
| 5 | VAL | LEU | ILE | ALA | GLN | PHE | THR | GLU | HIS | THR | ||||
| 6 | SER | ALA | ILE | LYS | VAL | ARG | GLY | LYS | ALA | TYR | ||||
| 7 | ILE | GLN | THR | ARG | HIS | GLY | VAL | ILE | GLU | SER | ||||
| 8 | GLU | GLY | LYS | LYS |
Entity 2, L-tryptophan - C11 H12 N2 O2 - 204.225 Da.
| 1 | TRP |
Samples:
A26I-TRAP-ILV: TRAP, [U-2H; U-15N; ILV-(13CH3)], 1-1.5 mM; potassium chloride 50 mM; sodium chloride 100 mM; sodium azide 0.02%
25C: pH: 8.0; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HSQC aliphatic | A26I-TRAP-ILV | isotropic | 25C |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
NMR spectrometers:
- Bruker Avance 800 MHz