BMRB Entry 17400

Title:
B. ste. A26I TRAP methyls
Deposition date:
2011-01-07
Original release date:
2012-02-13
Authors:
Kleckner, Ian
Citation:

Citation: Kleckner, Ian; Gollnick, Paul; Foster, Mark. "Mechanisms of allosteric gene regulation by NMR quantification of microsecond-millisecond protein dynamics"  J. Mol. Biol. 415, 372-381 (2012).
PubMed: 22115774

Assembly members:

Assembly members:
TRAP, polymer, 74 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus stearothermophilus   Taxonomy ID: 1422   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus stearothermophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts29
1H chemical shifts87

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TRAP, chain 11
2TRAP, chain 21
3TRAP, chain 31
4TRAP, chain 41
5TRAP, chain 51
6TRAP, chain 61
7TRAP, chain 71
8TRAP, chain 81
9TRAP, chain 91
10TRAP, chain 101
11TRAP, chain 111

Entities:

Entity 1, TRAP, chain 1 74 residues - Formula weight is not available

1   METTYRTHRASNSERASPPHEVALVALILE
2   LYSALALEUGLUASPGLYVALASNVALILE
3   GLYLEUTHRARGGLYILEASPTHRARGPHE
4   HISHISSERGLULYSLEUASPLYSGLYGLU
5   VALLEUILEALAGLNPHETHRGLUHISTHR
6   SERALAILELYSVALARGGLYLYSALATYR
7   ILEGLNTHRARGHISGLYVALILEGLUSER
8   GLUGLYLYSLYS

Samples:

A26I-TRAP-ILV: TRAP, [U-2H; U-15N; ILV-(13CH3)], 1-1.5 mM; potassium chloride 50 mM; sodium chloride 100 mM; sodium azide .02%

25C: pH: 8.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQC aliphaticA26I-TRAP-ILVisotropic25C

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 17413
PDB
DBJ BAD76498 GAD12442 GAJ58441
GB AAD33793 ABO67499 ACX77149 ADI26315 ADU94597
REF WP_008879621 WP_011231698 WP_033013997
SP Q9X6J6
AlphaFold Q9X6J6