BMRB Entry 17380

Name: rbp56 RanBP2 zinc finger backbone chemical shifts
Published: 2011-01-25

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17380

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

rbp56 RanBP2 zinc finger backbone chemical shifts

Deposition date:

2010-12-23

Original release date:

2011-01-25

Authors:

Mansfield, Robyn; Mackay, Joel; Nguyen, Cuong

Citation:

Citation: Nguyen, Cuong; Mansfield, Robyn; Leung, Wilfred; Vaz, Paula; Loughlin, Fionna; Grant, Richard; Mackay, Joel. "Characterization of a Family of RanBP2-Type Zinc Fingers that Can Recognize Single-Stranded RNA." J. Mol. Biol. 407, 273-283 (2011).
PubMed: 21256132

Assembly members:

Assembly members:
RBP56_ZnF, polymer, 42 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RBP56_ZnF: GSRGGDPKSGDWVCPNPSCG NMNFARRNSCNQCNEPRPED SR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	74
15N chemical shifts	32
1H chemical shifts	32

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	protein component	1
2	zinc ion	2

Entities:

Entity 1, protein component 42 residues - Formula weight is not available

N-terminal GS is non-native, resulting from cleavage of a GST tag.

1

GLY

SER

ARG

GLY

ASP

PRO

LYS

SER

GLY

2

ASP

TRP

VAL

CYS

PRO

ASN

PRO

SER

CYS

GLY

3

ASN

MET

ASN

PHE

ALA

ARG

ASN

SER

CYS

4

ASN

GLN

CYS

ASN

GLU

PRO

ARG

PRO

GLU

ASP

5

SER

ARG

Entity 2, zinc ion - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: RBP56 ZnF, [U-100% 13C; U-100% 15N], 1 mM; DTT 1 mM; sodium phosphate 25 mM; sodium chloride 100 mM; DSS 20 uM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.125 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker DRX 600 MHz

BMRB	19561
DBJ	BAA33811 BAA33812 BAB46889 BAC34676 BAE27086
EMBL	CAA67398
GB	AAC50932 AAH46099 AAI37592 AAI66769 AAO13485
REF	NP_001099294 NP_001302693 NP_003478 NP_081703 NP_631961
SP	Q92804
TPG	DAA19087
AlphaFold	Q92804