BMRB Entry 17378

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PDB ID: 2l7u
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17378
MolProbity Validation Chart

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Title:
Structure of CEL-PEP-RAGE V domain complex
Deposition date:
2010-12-23
Original release date:
2011-05-18
Authors:
Xue, Jing; Rai, Vivek; Schmidt, Ann-Marie; Frolov, Sergey; Reverdatto, Sergey; Singer, David; Chabierski, Stefan; Xie, Jingjing; Burz, David; Shekhtman, Alexander; Hoffman, Ralf
Citation:

Citation: Xue, Jing; Rai, Vivek; Singer, David; Chabierski, Stefan; Xie, Jingjing; Reverdatto, Sergey; Burz, David; Schmidt, Ann Marie; Hoffmann, Ralf; Shekhtman, Alexander. "Advanced Glycation End Product Recognition by the Receptor for AGEs."  Structure 19, 722-732 (2011).
PubMed: 21565706

Assembly members:

Assembly members:
V_domain, polymer, 105 residues, 11571.429 Da.
CEL-PEP, polymer, 7 residues, 724.677 Da.
MCL, polymer, . residues, 218.250 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
V_domain: GSAQNITARIGEPLVLKCKG APKKPPQRLEWKLNTGRTEA WKVLSPQGGGPWDSVARVLP NGSLFLPAVGIQDEGIFRCQ AMNRNGKETKSNYRVRVYQI PGKPE
CEL-PEP: DEFXADE

Data sets:
Data typeCount
13C chemical shifts345
15N chemical shifts92
1H chemical shifts656

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1v_domain1
2CEL-PEP2
34-[5-(4-METHYLPHENYL)-3-(TRIFLUOROMETHYL)-1H-PYRAZOL-1-YL]BENZENESULFONAMIDE3

Entities:

Entity 1, v_domain 105 residues - 11571.429 Da.

Residues 1-2 represent non-native affinity tag Residues 3-105 in the PDB file represent residues 23-123 of the V domain of RAGE.

1   GLYSERALAGLNASNILETHRALAARGILE
2   GLYGLUPROLEUVALLEULYSCYSLYSGLY
3   ALAPROLYSLYSPROPROGLNARGLEUGLU
4   TRPLYSLEUASNTHRGLYARGTHRGLUALA
5   TRPLYSVALLEUSERPROGLNGLYGLYGLY
6   PROTRPASPSERVALALAARGVALLEUPRO
7   ASNGLYSERLEUPHELEUPROALAVALGLY
8   ILEGLNASPGLUGLYILEPHEARGCYSGLN
9   ALAMETASNARGASNGLYLYSGLUTHRLYS
10   SERASNTYRARGVALARGVALTYRGLNILE
11   PROGLYLYSPROGLU

Entity 2, CEL-PEP 7 residues - 724.677 Da.

This sequence contains a modified lysine, Nepsilon -carboxy-ethyl-lysine (CEL). The CEL is denoted by X.

1   ASPGLUPHECELALAASPGLU

Entity 3, 4-[5-(4-METHYLPHENYL)-3-(TRIFLUOROMETHYL)-1H-PYRAZOL-1-YL]BENZENESULFONAMIDE - 218.250 Da.

1   MCL

Related Database Links:

BMRB 19739 19953
PDB 2E5E 2L7U 2M1K 2MJW 2MOV 3CJJ 3O3U 4LP4 4LP5 4OF5 4OFV 4OI7 4OI8 4P2Y
DBJ BAA05958 BAA89369 BAC65465 BAG35995 BAG60385
GB AAA03574 AAB47491 AAH20669 AAX07272 AAX07273
REF NP_001127 NP_001192046 NP_001193858 NP_001193863 NP_001193865
SP Q15109
AlphaFold Q15109 Q3SZR2

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