BMRB Entry 17367

Name: Assignments of Drosophila cytotoxic T-lymphocyte antigen 2 (CTLA-2) like protein, crammer at pH3
Published: 2012-07-25

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17367

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assignments of Drosophila cytotoxic T-lymphocyte antigen 2 (CTLA-2) like protein, crammer at pH3

Deposition date:

2010-12-19

Original release date:

2012-07-25

Authors:

Tzeng, Tien-Sheng; Hsu, Shang-Te Danny; Lyu, Ping-Chiang

Citation:

Citation: Tseng, Tien-Sheng; Cheng, Chao-Sheng; Chen, Dian-Jiun; Shih, Min-Fang; Liu, Yu-Nan; Hsu, Shang-Te; Lyu, Ping-Chiang. "A Molten Globule-to-Ordered Structure Transition of Drosophila melanogaster Crammer Is Required for its Ability to Inhibit Cathepsin" Biochem. J. 442, 563-572 (2012).
PubMed: 22150223

Assembly members:

Assembly members:
C72S_monomer, polymer, 80 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pAED4

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C72S_monomer: MMSLVSDEEWVEYKSKFDKN YEAEEDLMRRRIYAESKARI EEHNRKFEKGEVTWKMGINH LADLTPEEFAQRSGKKVPPN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	224
15N chemical shifts	74
1H chemical shifts	73

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C72S monomer	1

Entities:

Entity 1, C72S monomer 80 residues - Formula weight is not available

1	MET	MET	SER	LEU	VAL	SER	ASP	GLU	GLU	TRP
2	VAL	GLU	TYR	LYS	SER	LYS	PHE	ASP	LYS	ASN
3	TYR	GLU	ALA	GLU	GLU	ASP	LEU	MET	ARG	ARG
4	ARG	ILE	TYR	ALA	GLU	SER	LYS	ALA	ARG	ILE
5	GLU	GLU	HIS	ASN	ARG	LYS	PHE	GLU	LYS	GLY
6	GLU	VAL	THR	TRP	LYS	MET	GLY	ILE	ASN	HIS
7	LEU	ALA	ASP	LEU	THR	PRO	GLU	GLU	PHE	ALA
8	GLN	ARG	SER	GLY	LYS	LYS	VAL	PRO	PRO	ASN

Samples:

sample_1: C72S_monomer, [U-98% 13C; U-98% 15N], 0.5 mM

sample_conditions_1: pH: 3; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

SPARKY_and_NMRpipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - chemical shift assignment, processing

NMR spectrometers:

Varian INOVA 700 MHz

BMRB	16719
PDB	2L95
GB	AAF57567 ACJ13252 EDV55205 EDW48595 EDX07811
REF	NP_611420 XP_001974805 XP_002034582 XP_002082226

1	MET	MET	SER	LEU	VAL	SER	ASP	GLU	GLU	TRP
2	VAL	GLU	TYR	LYS	SER	LYS	PHE	ASP	LYS	ASN
3	TYR	GLU	ALA	GLU	GLU	ASP	LEU	MET	ARG	ARG
4	ARG	ILE	TYR	ALA	GLU	SER	LYS	ALA	ARG	ILE
5	GLU	GLU	HIS	ASN	ARG	LYS	PHE	GLU	LYS	GLY
6	GLU	VAL	THR	TRP	LYS	MET	GLY	ILE	ASN	HIS
7	LEU	ALA	ASP	LEU	THR	PRO	GLU	GLU	PHE	ALA
8	GLN	ARG	SER	GLY	LYS	LYS	VAL	PRO	PRO	ASN

1	MET	MET	SER	LEU	VAL	SER	ASP	GLU	GLU	TRP
2	VAL	GLU	TYR	LYS	SER	LYS	PHE	ASP	LYS	ASN
3	TYR	GLU	ALA	GLU	GLU	ASP	LEU	MET	ARG	ARG
4	ARG	ILE	TYR	ALA	GLU	SER	LYS	ALA	ARG	ILE
5	GLU	GLU	HIS	ASN	ARG	LYS	PHE	GLU	LYS	GLY
6	GLU	VAL	THR	TRP	LYS	MET	GLY	ILE	ASN	HIS
7	LEU	ALA	ASP	LEU	THR	PRO	GLU	GLU	PHE	ALA
8	GLN	ARG	SER	GLY	LYS	LYS	VAL	PRO	PRO	ASN