BMRB Entry 16719

Name: Solution structure of Cytotoxic T-Lymphocyte Antigent-2(CTLA-2) like protein, Crammer
Published: 2011-12-20

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PDB ID: 2l95
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16719
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of Cytotoxic T-Lymphocyte Antigent-2(CTLA-2) like protein, Crammer

Deposition date:

2010-02-11

Original release date:

2011-12-20

Authors:

Tzeng, Tain-Sheng; Cheng, Chao-Sheng; Liu, Yu-Nan; Shih, Min-fang; Lyu, Ping-Chiang

Citation:

Citation: Tseng, Tien-Sheng; Cheng, Chao-Sheng; Chen, Dian-Jiun; Shih, Min-Fang; Liu, Yu-Nan; Hsu, Shang-Te Danny; Lyu, Ping-Chiang. "A molten globule-to-ordered structure transition of Drosophila melanogaster crammer is required for its ability to inhibit cathepsin." Biochem. J. 442, 563-572 (2012).
PubMed: 22150223

Assembly members:

Assembly members:
Crammer, polymer, 80 residues, 9595.900 Da.

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PAED4

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Crammer: MMSLVSDEEWVEYKSKFDKN YEAEEDLMRRRIYAESKARI EEHNRKFEKGEVTWKMGINH LADLTPEEFAQRSGKKVPPN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	215
15N chemical shifts	73
1H chemical shifts	351

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Crammer	1

Entities:

Entity 1, Crammer 80 residues - 9595.900 Da.

1	MET	MET	SER	LEU	VAL	SER	ASP	GLU	GLU	TRP
2	VAL	GLU	TYR	LYS	SER	LYS	PHE	ASP	LYS	ASN
3	TYR	GLU	ALA	GLU	GLU	ASP	LEU	MET	ARG	ARG
4	ARG	ILE	TYR	ALA	GLU	SER	LYS	ALA	ARG	ILE
5	GLU	GLU	HIS	ASN	ARG	LYS	PHE	GLU	LYS	GLY
6	GLU	VAL	THR	TRP	LYS	MET	GLY	ILE	ASN	HIS
7	LEU	ALA	ASP	LEU	THR	PRO	GLU	GLU	PHE	ALA
8	GLN	ARG	SER	GLY	LYS	LYS	VAL	PRO	PRO	ASN

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_3	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_3	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_3	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HN(CO)CA	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1

BMRB	17367
PDB	2L95
GB	AAF57567 ACJ13252 EDV55205 EDW48595 EDX07811
REF	NP_611420 XP_001974805 XP_002034582 XP_002082226

BMRB Entry 16719

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	MET	SER	LEU	VAL	SER	ASP	GLU	GLU	TRP
2	VAL	GLU	TYR	LYS	SER	LYS	PHE	ASP	LYS	ASN
3	TYR	GLU	ALA	GLU	GLU	ASP	LEU	MET	ARG	ARG
4	ARG	ILE	TYR	ALA	GLU	SER	LYS	ALA	ARG	ILE
5	GLU	GLU	HIS	ASN	ARG	LYS	PHE	GLU	LYS	GLY
6	GLU	VAL	THR	TRP	LYS	MET	GLY	ILE	ASN	HIS
7	LEU	ALA	ASP	LEU	THR	PRO	GLU	GLU	PHE	ALA
8	GLN	ARG	SER	GLY	LYS	LYS	VAL	PRO	PRO	ASN

1	MET	MET	SER	LEU	VAL	SER	ASP	GLU	GLU	TRP
2	VAL	GLU	TYR	LYS	SER	LYS	PHE	ASP	LYS	ASN
3	TYR	GLU	ALA	GLU	GLU	ASP	LEU	MET	ARG	ARG
4	ARG	ILE	TYR	ALA	GLU	SER	LYS	ALA	ARG	ILE
5	GLU	GLU	HIS	ASN	ARG	LYS	PHE	GLU	LYS	GLY
6	GLU	VAL	THR	TRP	LYS	MET	GLY	ILE	ASN	HIS
7	LEU	ALA	ASP	LEU	THR	PRO	GLU	GLU	PHE	ALA
8	GLN	ARG	SER	GLY	LYS	LYS	VAL	PRO	PRO	ASN

1	MET	MET	SER	LEU	VAL	SER	ASP	GLU	GLU	TRP
2	VAL	GLU	TYR	LYS	SER	LYS	PHE	ASP	LYS	ASN
3	TYR	GLU	ALA	GLU	GLU	ASP	LEU	MET	ARG	ARG
4	ARG	ILE	TYR	ALA	GLU	SER	LYS	ALA	ARG	ILE
5	GLU	GLU	HIS	ASN	ARG	LYS	PHE	GLU	LYS	GLY
6	GLU	VAL	THR	TRP	LYS	MET	GLY	ILE	ASN	HIS
7	LEU	ALA	ASP	LEU	THR	PRO	GLU	GLU	PHE	ALA
8	GLN	ARG	SER	GLY	LYS	LYS	VAL	PRO	PRO	ASN