BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17343

Title: Structure of the NOXO1b PX domain   PubMed: 21188560

Deposition date: 2010-12-02 Original release date: 2011-03-30

Authors: Davis, Nicole; McPhail, Linda; Horita, David

Citation: Davis, Nicole; McPhail, Linda; Horita, David. "Backbone 1H, 15N, and 13C resonance assignments for the NOXO1 PX domain."  Biomol. NMR Assignments 5, 139-141 (2011).

Assembly members:
NOXO1b, polymer, 149 residues, 16060.686 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology' 'Escherichia coli

Entity Sequences (FASTA):
NOXO1b: GPLGSMAGPRYPVSVQGAAL VQIKRLQTFAFSVRWSDGSD TFVRRSWDEFRQLKKTLKET FPVEAGLLRRSDRVLPKLLD APLLGRVGRTSRGLARLQLL ETYSRRLLATAERVARSPTI TGFFAPQPLDLEPALPPGSR VILPTPEEQ

Data sets:
Data typeCount
13C chemical shifts443
15N chemical shifts135
1H chemical shifts341

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NOXO1b1

Entities:

Entity 1, NOXO1b 149 residues - 16060.686 Da.

Residues 1-5 are remnants of N-terminal fusion tag

1   GLYPROLEUGLYSERMETALAGLYPROARG
2   TYRPROVALSERVALGLNGLYALAALALEU
3   VALGLNILELYSARGLEUGLNTHRPHEALA
4   PHESERVALARGTRPSERASPGLYSERASP
5   THRPHEVALARGARGSERTRPASPGLUPHE
6   ARGGLNLEULYSLYSTHRLEULYSGLUTHR
7   PHEPROVALGLUALAGLYLEULEUARGARG
8   SERASPARGVALLEUPROLYSLEULEUASP
9   ALAPROLEULEUGLYARGVALGLYARGTHR
10   SERARGGLYLEUALAARGLEUGLNLEULEU
11   GLUTHRTYRSERARGARGLEULEUALATHR
12   ALAGLUARGVALALAARGSERPROTHRILE
13   THRGLYPHEPHEALAPROGLNPROLEUASP
14   LEUGLUPROALALEUPROPROGLYSERARG
15   VALILELEUPROTHRPROGLUGLUGLN

Samples:

sample_1: NOXO1b, [U-13C; U-15N; U-2H], 0.5 ± 0.05 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 293 K

sample_conditions_2: pH: 6.5; temperature: 293 K

sample_conditions_3: pH: 6.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D IPAPsample_1anisotropicsample_conditions_2
2D IPAPsample_1anisotropicsample_conditions_3
3D HNCOsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HNCOCAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOCACBsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HMCGCBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYHSQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESYHSQCsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 16749
PDB
DBJ BAC76711 BAF84886 BAI47049
GB AAH15917 AAM97925 AAM97926 AAN75141 AAP13479
REF NP_653204 NP_751907 XP_008958072 XP_012352685 XP_012352686

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts