BMRB Entry 16749

Name: Backbone Assignments of the PX Domain of Noxo1b
Published: 2011-03-08

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16749

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone Assignments of the PX Domain of Noxo1b

Deposition date:

2010-03-02

Original release date:

2011-03-08

Authors:

Davis, Nicole; Horita, David; McPhail, Linda

Citation:

Citation: Davis, Nicole; McPhail, Linda; Horita, David. "Backbone 1H, 15N, and 13C resonance assignments for the NOXO1 PX domain." Biomol. NMR Assignments 5, 139-141 (2011).
PubMed: 21188560

Assembly members:

Assembly members:
Noxo1b_PX, polymer, 149 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX6P-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Noxo1b_PX: GPLGSMAGPRYPVSVQGAAL VQIKRLQTFAFSVRWSDGSD TFVRRSWDEFRQLKKTLKET FPVEAGLLRRSDRVLPKLLD APLLGRVGRTSRGLARLQLL ETYSRRLLATAERVARSPTI TGFFAPQPLDLEPALPPGSR VILPTPEEQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	406
15N chemical shifts	128
1H chemical shifts	128

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Noxo1b PX	1

Entities:

Entity 1, Noxo1b PX 149 residues - Formula weight is not available

residues 1-5 are non-native affinity tag

1

GLY

PRO

LEU

GLY

SER

MET

ALA

GLY

PRO

ARG

2

TYR

PRO

VAL

SER

VAL

GLN

GLY

ALA

LEU

3

VAL

GLN

ILE

LYS

ARG

LEU

GLN

THR

PHE

ALA

4

PHE

SER

VAL

ARG

TRP

SER

ASP

GLY

SER

ASP

5

THR

PHE

VAL

ARG

SER

TRP

ASP

GLU

PHE

6

ARG

GLN

LEU

LYS

THR

LEU

LYS

GLU

THR

7

PHE

PRO

VAL

GLU

ALA

GLY

LEU

ARG

8

SER

ASP

ARG

VAL

LEU

PRO

LYS

LEU

ASP

9

ALA

PRO

LEU

GLY

ARG

VAL

GLY

ARG

THR

10

SER

ARG

GLY

LEU

ALA

ARG

LEU

GLN

LEU

11

GLU

THR

TYR

SER

ARG

LEU

ALA

THR

12

ALA

GLU

ARG

VAL

ALA

ARG

SER

PRO

THR

ILE

13

THR

GLY

PHE

ALA

PRO

GLN

PRO

LEU

ASP

14

LEU

GLU

PRO

ALA

LEU

PRO

GLY

SER

ARG

15

VAL

ILE

LEU

PRO

THR

PRO

GLU

GLN

Samples:

sample_1: Noxo1b PX, [U-13C; U-15N; U-2H], 500 uM; sodium phosphate 100 mM; sodium chloride 100 mM; EDTA 0.1 mM; H2O 90%; glycerol 5%; D2O 10%

sample_conditions_1: ionic strength: 100 mM; pH: 6.9; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

Bruker Avance 600 MHz

BMRB	17343
PDB	2L73
DBJ	BAC76711 BAF84886 BAI47049
GB	AAH15917 AAM97925 AAM97926 AAN75141 AAP13479
REF	NP_653204 NP_751907 XP_008958072 XP_012352685 XP_012352686