BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16749

Title: Backbone Assignments of the PX Domain of Noxo1b   PubMed: 21188560

Deposition date: 2010-03-02 Original release date: 2011-03-08

Authors: Davis, Nicole; Horita, David; McPhail, Linda

Citation: Davis, Nicole; McPhail, Linda; Horita, David. "Backbone 1H, 15N, and 13C resonance assignments for the NOXO1 PX domain."  Biomol. NMR Assignments 5, 139-141 (2011).

Assembly members:
Noxo1b_PX, polymer, 149 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology' 'Escherichia coli

Entity Sequences (FASTA):
Noxo1b_PX: GPLGSMAGPRYPVSVQGAAL VQIKRLQTFAFSVRWSDGSD TFVRRSWDEFRQLKKTLKET FPVEAGLLRRSDRVLPKLLD APLLGRVGRTSRGLARLQLL ETYSRRLLATAERVARSPTI TGFFAPQPLDLEPALPPGSR VILPTPEEQ

Data sets:
Data typeCount
13C chemical shifts406
15N chemical shifts128
1H chemical shifts128

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Noxo1b PX1

Entities:

Entity 1, Noxo1b PX 149 residues - Formula weight is not available

residues 1-5 are non-native affinity tag

1   GLYPROLEUGLYSERMETALAGLYPROARG
2   TYRPROVALSERVALGLNGLYALAALALEU
3   VALGLNILELYSARGLEUGLNTHRPHEALA
4   PHESERVALARGTRPSERASPGLYSERASP
5   THRPHEVALARGARGSERTRPASPGLUPHE
6   ARGGLNLEULYSLYSTHRLEULYSGLUTHR
7   PHEPROVALGLUALAGLYLEULEUARGARG
8   SERASPARGVALLEUPROLYSLEULEUASP
9   ALAPROLEULEUGLYARGVALGLYARGTHR
10   SERARGGLYLEUALAARGLEUGLNLEULEU
11   GLUTHRTYRSERARGARGLEULEUALATHR
12   ALAGLUARGVALALAARGSERPROTHRILE
13   THRGLYPHEPHEALAPROGLNPROLEUASP
14   LEUGLUPROALALEUPROPROGLYSERARG
15   VALILELEUPROTHRPROGLUGLUGLN

Samples:

sample_1: Noxo1b PX' '[U-13C; U-15N; U-2H]; .; . mM; . mM; . %; . %; . %

sample_conditions_1: ionic strength: 100 mM; pH: 6.9; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 17343
PDB
DBJ BAC76711 BAF84886 BAI47049
GB AAH15917 AAM97925 AAM97926 AAN75141 AAP13479
REF NP_653204 NP_751907 XP_008958072 XP_012352685 XP_012352686

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts