BMRB Entry 17096
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17096
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Citation: Hoeltzli, Sydney; Frieden, Carl. "Stopped-flow NMR spectroscopy: Real-time unfolding studies of 6-19F-tryptophan-labeled Escherichia coli dihydrofolate reductase" Proc. Natl. Acad. Sci. U.S.A. 92, 9318-9322 (1995).
PubMed: 7568125
Assembly members:
DHFR, polymer, 187 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMONDHFR
- chemical_rates
| Data type | Count |
| kinetic rates | 1 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DHFR | 1 |
Entities:
Entity 1, DHFR 187 residues - Formula weight is not available
| 1 | MET | VAL | GLY | SER | LEU | ASN | CYS | ILE | VAL | ALA | ||||
| 2 | VAL | SER | GLN | ASN | MET | GLY | ILE | GLY | LYS | ASN | ||||
| 3 | GLY | ASP | LEU | PRO | TRP | PRO | PRO | LEU | ARG | ASN | ||||
| 4 | GLU | PHE | ARG | TYR | PHE | GLN | ARG | MET | THR | THR | ||||
| 5 | THR | SER | SER | VAL | GLU | GLY | LYS | GLN | ASN | LEU | ||||
| 6 | VAL | ILE | MET | GLY | LYS | LYS | THR | TRP | PHE | SER | ||||
| 7 | ILE | PRO | GLU | LYS | ASN | ARG | PRO | LEU | LYS | GLY | ||||
| 8 | ARG | ILE | ASN | LEU | VAL | LEU | SER | ARG | GLU | LEU | ||||
| 9 | LYS | GLU | PRO | PRO | GLN | GLY | ALA | HIS | PHE | LEU | ||||
| 10 | SER | ARG | SER | LEU | ASP | ASP | ALA | LEU | LYS | LEU | ||||
| 11 | THR | GLU | GLN | PRO | GLU | LEU | ALA | ASN | LYS | VAL | ||||
| 12 | ASP | MET | VAL | TRP | ILE | VAL | GLY | GLY | SER | SER | ||||
| 13 | VAL | TYR | LYS | GLU | ALA | MET | ASN | HIS | PRO | GLY | ||||
| 14 | HIS | LEU | LYS | LEU | PHE | VAL | THR | ARG | ILE | MET | ||||
| 15 | GLN | ASP | PHE | GLU | SER | ASP | THR | PHE | PHE | PRO | ||||
| 16 | GLU | ILE | ASP | LEU | GLU | LYS | TYR | LYS | LEU | LEU | ||||
| 17 | PRO | GLU | TYR | PRO | GLY | VAL | LEU | SER | ASP | VAL | ||||
| 18 | GLN | GLU | GLU | LYS | GLY | ILE | LYS | TYR | LYS | PHE | ||||
| 19 | GLU | VAL | TYR | GLU | LYS | ASN | ASP |
Samples:
sample_1: potassium phosphate 50 mM; EDTA 0.1 mM; DTT 3 mM; sodium azide 5 mM; DHFR, [U-100% 19F-tryptophan], 0.89 mM; urea0 – 5 M; H2O 85%; D2O 15%
sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 295 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 19F NMR | sample_1 | isotropic | sample_conditions_1 |
Software:
KaleidaGraph, Synergy Software, Reading, PA - exponential fits
VNMR, Varian - data analysis
NMR spectrometers:
- Varian VXR-500 470.3 MHz