BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19565

Title: chemical shift assignments and 3Jcgn & 3Jcgc' scalar couplings for human dihdyrofolate reductase bound to NADP+ and THF   PubMed: 24498949

Deposition date: 2013-10-18 Original release date: 2016-04-04

Authors: Wright, Peter; ., Bhabha; M., .; ., J.

Citation: Tuttle, Lisa; ., Dyson; E., .. "Side Chain Conformational Averaging in Human Dihydrofolate Reductase"  Biochemistry 53, 1134-1145 (2014).

Assembly members:
DHFR, polymer, 187 residues, Formula weight is not available
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, non-polymer, 745.421 Da.
(6S)-5,6,7,8-TETRAHYDROFOLATE, non-polymer, 445.429 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology' 'Escherichia coli

Entity Sequences (FASTA):
DHFR: MVGSLNCIVAVSQNMGIGKN GDLPWPPLRNEFRYFQRMTT TSSVEGKQNLVIMGKKTWFS IPEKNRPLKGRINLVLSREL KEPPQGAHFLSRSLDDALKL TEQPELANKVDMVWIVGGSS VYKEAMNHPGHLKLFVTRIM QDFESDTFFPEIDLEKYKLL PEYPGVLSDVQEEKGIKYKF EVYEKND

Data sets:
Data typeCount
13C chemical shifts260
1701H chemical shifts
coupling constants122

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