BMRB Entry 17068

Name: Assigment of the 1H, 13C, and 15N resonances of the yeast frataxin (Yfh1) under cold denaturation
Published: 2010-11-09

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17068

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assigment of the 1H, 13C, and 15N resonances of the yeast frataxin (Yfh1) under cold denaturation

Deposition date:

2010-07-21

Original release date:

2010-11-09

Authors:

Adrover, Miquel; Pastore, Annalisa; Temussi, Piero Andrea

Citation:

Citation: Adrover, Miquel; Esposito, Veronica; Martorell, Gabriel; Pastore, Annalisa; Temussi, Piero Andrea. "Understanding cold denaturation: the case study of yfh1." J. Am. Chem. Soc. 132, 16240-16246 (2010).
PubMed: 20979399

Assembly members:

Assembly members:
Yeast_frataxin_(Yfh1), polymer, 123 residues, 13783.4 Da.

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: Escherichia coli Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Yeast_frataxin_(Yfh1): MESSTDGQVVPQEVLNLPLE KYHEEADDYLDHLLDSLEEL SEAHPDCIPDVELSHGVMTL EIPAFGTYVINKQPPNKQIW LASPLSGPNRFDLLNGEWVS LRNGTKLTDILTEEVEKAIS KSQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	210
15N chemical shifts	92
1H chemical shifts	592

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Frataxin	1

Entities:

Entity 1, Frataxin 123 residues - 13783.4 Da.

1

MET

GLU

SER

THR

ASP

GLY

GLN

VAL

2

PRO

GLN

GLU

VAL

LEU

ASN

LEU

PRO

LEU

GLU

3

LYS

TYR

HIS

GLU

ALA

ASP

TYR

LEU

4

ASP

HIS

LEU

ASP

SER

LEU

GLU

LEU

5

SER

GLU

ALA

HIS

PRO

ASP

CYS

ILE

PRO

ASP

6

VAL

GLU

LEU

SER

HIS

GLY

VAL

MET

THR

LEU

7

GLU

ILE

PRO

ALA

PHE

GLY

THR

TYR

VAL

ILE

8

ASN

LYS

GLN

PRO

ASN

LYS

GLN

ILE

TRP

9

LEU

ALA

SER

PRO

LEU

SER

GLY

PRO

ASN

ARG

10

PHE

ASP

LEU

ASN

GLY

GLU

TRP

VAL

SER

11

LEU

ARG

ASN

GLY

THR

LYS

LEU

THR

ASP

ILE

12

LEU

THR

GLU

VAL

GLU

LYS

ALA

ILE

SER

13

LYS

SER

GLN

Samples:

sample_1: Yeast frataxin (Yfh1), [U-100% 13C; U-100% 15N], 0.4 – 0.5 mM; D2O 90 ± 2 %; H2O 10 ± 2 %; DTT 1 ± 0.2 mM; HEPES 20 ± 1 mM

sample_conditions_1: ionic strength: 0.004 M; pH: 7.0; pressure: 1 atm; temperature: 272.0 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

XEASY v1.3.2, Bartels et al., Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, peak picking, processing

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	6356 17641 19991
PDB	2fql 2ga5 2FQL 2GA5 3OEQ 3OER 4EC2
DBJ	GAA22128
EMBL	CAA98688 CAY78388
GB	AAS56486 AHY74893 AJP37633 AJU57746 AJU58449
REF	NP_010163
SP	Q07540
TPG	DAA11740
AlphaFold	Q07540