BMRB Entry 17009

Name: Sequence specific backbone assignment of the C-terminal domain of MqsA
Published: 2010-12-03

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17009

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Sequence specific backbone assignment of the C-terminal domain of MqsA

Deposition date:

2010-06-19

Original release date:

2010-12-03

Authors:

Brown, Breann; Grigoriu, Simina; Arruda, Jennifer; Page, Rebecca; Peti, Wolfgang

Citation:

Citation: Brown, Breann; Wood, Thomas; Peti, Wolfgang; Page, Rebecca. "Structure of the Escherichia coli Antitoxin MqsA (YgiT/b3021) Bound to Its Gene Promoter Reveals Extensive Domain Rearrangements and the Specificity of Transcriptional Regulation." J. Biol. Chem. 286, 2285-2296 (2011).

Assembly members:

Assembly members:
mqsa_c, polymer, 73 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: RP1B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
mqsa_c: GHMASVNAETVAPEFIVKVR KKLSLTQKEASEIFGGGVNA FSRYEKGNAQPHPSTIKLLR VLDKHPELLNEIR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	288
15N chemical shifts	65
1H chemical shifts	65

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	mqsa cterm	1

Entities:

Entity 1, mqsa cterm 73 residues - Formula weight is not available

GHM cloning artifacts; Alanine is residue 62 of MqsA

1

GLY

HIS

MET

ALA

SER

VAL

ASN

ALA

GLU

THR

2

VAL

ALA

PRO

GLU

PHE

ILE

VAL

LYS

VAL

ARG

3

LYS

LEU

SER

LEU

THR

GLN

LYS

GLU

ALA

4

SER

GLU

ILE

PHE

GLY

VAL

ASN

ALA

5

PHE

SER

ARG

TYR

GLU

LYS

GLY

ASN

ALA

GLN

6

PRO

HIS

PRO

SER

THR

ILE

LYS

LEU

ARG

7

VAL

LEU

ASP

LYS

HIS

PRO

GLU

LEU

ASN

8

GLU

ILE

ARG

Samples:

sample_1: mqsa_c, [U-99% 15N], 0.5 mM; sodium chloride 50 mM; TRIS 10 mM; H2O 90%; D2O 10%

sample_2: mqsa_c, [U-99% 13C; U-99% 15N], 0.5 mM; sodium chloride 50 mM; TRIS 10 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 60 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D C(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

XEASY v1.8.2, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

Bruker Avance 500 MHz

BMRB	15446
PDB	2KZ8 3FMY 3GN5 3O9X
DBJ	BAE77077 BAJ44773 BAL39680
EMBL	CAQ33360 CAR14662 CAR19632 CBJ02792 CCJ45634
GB	AAA69189 AAC76057 ABV07432 ACA76352 ACB04106
REF	NP_417493 WP_000650107 WP_001487520 WP_021572968 WP_044814339
SP	Q46864
AlphaFold	Q46864