BMRB Entry 15446

Name: 2cxxc, YgiT
Published: 2008-01-30

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15446

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

2cxxc, YgiT

Deposition date:

2007-08-28

Original release date:

2008-01-30

Authors:

Papadopoulos, Evangelos; Gardikas Vlamis, Alexios; Graslund, Astrid; Billeter, Martin

Citation:

Citation: Papadopoulos, Evangelos; Billeter, Martin; Graslund, Astrid; Vlamis-Gardikas, Alexios. "Assignment of (1)H, (13)C, and (15)N resonances of YgiT, a putative DNA interacting protein from E. coli, containing one HTH and two CxxC motifs" Biomol. NMR Assignments 1, 217-219 (2007).
PubMed: 19636869

Assembly members:

Assembly members:
2cxxc_-_Ygit, polymer, 131 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
2cxxc_-_Ygit: MKCPVCHQGEMVSGIKDIPY TFRGRKTVLKGIHGLYCVHC EESIMNKEESDAFMAQVKAF RASVNAETVAPEFIVKVRKK LSLTQKEASEIFGGGVNAFS RYEKGNAQPHPSTIKLLRVL DKHPELLNEIR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	510
15N chemical shifts	127
1H chemical shifts	685

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	2cxxc	1
2	Zinc ion	2

Entities:

Entity 1, 2cxxc 131 residues - Formula weight is not available

1

MET

LYS

CYS

PRO

VAL

CYS

HIS

GLN

GLY

GLU

2

MET

VAL

SER

GLY

ILE

LYS

ASP

ILE

PRO

TYR

3

THR

PHE

ARG

GLY

ARG

LYS

THR

VAL

LEU

LYS

4

GLY

ILE

HIS

GLY

LEU

TYR

CYS

VAL

HIS

CYS

5

GLU

SER

ILE

MET

ASN

LYS

GLU

SER

6

ASP

ALA

PHE

MET

ALA

GLN

VAL

LYS

ALA

PHE

7

ARG

ALA

SER

VAL

ASN

ALA

GLU

THR

VAL

ALA

8

PRO

GLU

PHE

ILE

VAL

LYS

VAL

ARG

LYS

9

LEU

SER

LEU

THR

GLN

LYS

GLU

ALA

SER

GLU

10

ILE

PHE

GLY

VAL

ASN

ALA

PHE

SER

11

ARG

TYR

GLU

LYS

GLY

ASN

ALA

GLN

PRO

HIS

12

PRO

SER

THR

ILE

LYS

LEU

ARG

VAL

LEU

13

ASP

LYS

HIS

PRO

GLU

LEU

ASN

GLU

ILE

14

ARG

Entity 2, Zinc ion - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: 2cxxc - Ygit, [U-100% 13C; U-100% 15N], 1 mM; Zinc ion 1 mM; Sodium Phosphate buffer 20 mM

sample_conditions_1: ionic strength: 25 mM; pH: 5; pressure: 0.7 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian INOVA 800 MHz

BMRB	17009
PDB	2KZ8 3FMY 3GA8 3GN5 3HI2 3O9X
DBJ	BAE77077 BAJ44773 BAL39680
EMBL	CAQ33360 CAR14662 CAR19632 CBJ02792 CCJ45634
GB	AAA69189 AAC76057 ABV07432 ACA76352 ACB04106
REF	NP_417493 WP_000650107 WP_001487520 WP_021572968 WP_044814339
SP	Q46864
AlphaFold	Q46864