BMRB Entry 17001

Name: ASIP(80-132, P103A, P105A, Q115Y, S124Y)
Published: 2010-09-24

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PDB ID: 2kza
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17001
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

ASIP(80-132, P103A, P105A, Q115Y, S124Y)

Deposition date:

2010-06-14

Original release date:

2010-09-24

Authors:

Patel, Mira; Cribb Fabersunne, Camila; Yang, Ying-kui; Kaelin, Chris; Barsh, Gregory; Millhauser, Glenn

Citation:

Citation: Patel, Mira; Cribb Fabersunne, Camila; Yang, Ying-Kui; Kaelin, Christopher; Barsh, Gregory; Millhauser, Glenn. "Loop-swapped chimeras of the agouti-related protein and the agouti signaling protein identify contacts required for melanocortin 1 receptor selectivity and antagonism." J. Mol. Biol. 404, 45-55 (2010).
PubMed: 20831872

Assembly members:

Assembly members:
ASIP(80-132,_P103A,_P105A,_Q115Y,_S124Y), polymer, 53 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ASIP(80-132,_P103A,_P105A,_Q115Y,_S124Y): KKVVRPRTPLSAPCVATRNS CKPAAAACCDPCASCYCRFF RSACYCRVLSLNC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	303

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ASIP(80-132,_P103A,_P105A,_Q115Y,_S124Y)	1

Entities:

Entity 1, ASIP(80-132,_P103A,_P105A,_Q115Y,_S124Y) 53 residues - Formula weight is not available

1

LYS

VAL

ARG

PRO

ARG

THR

PRO

LEU

2

SER

ALA

PRO

CYS

VAL

ALA

THR

ARG

ASN

SER

3

CYS

LYS

PRO

ALA

CYS

ASP

4

PRO

CYS

ALA

SER

CYS

TYR

CYS

ARG

PHE

5

ARG

SER

ALA

CYS

TYR

CYS

ARG

VAL

LEU

SER

6

LEU

ASN

CYS

Samples:

sample_1: ASIP(80-132, P103A, P105A, Q115Y, S124Y) 1-2 mM; D2O 10%; H2O 90%; acetic acid, [U-100% 2H], 50 mM; sodium azide .1 % w/v; TSP 200 uM

sample_2: ASIP(80-132, P103A, P105A, Q115Y, S124Y) 1-2 mM; D2O, [U-100% 2H], 100%; acetic acid, [U-100% 2H], 50 mM; sodium azide .1 % w/v; TSP 200 uM

sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 298 K

sample_conditions_2: pH: 4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

Bruker Avance 900 MHz
Varian INOVA 600 MHz