BMRB Entry 17086

Name: 1H assignments for ASIP(93-126, P103A, P105A, P111A, Q115Y, S124Y)
Published: 2011-10-28

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PDB ID: 2l1j
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17086
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H assignments for ASIP(93-126, P103A, P105A, P111A, Q115Y, S124Y)

Deposition date:

2010-07-28

Original release date:

2011-10-28

Authors:

Patel, Mira; Cribb Fabersunne, Camila; Yang, Ying-kui; Kaelin, Chris; Barsh, Gregory; Millhauser, Glenn

Citation:

Citation: Patel, Mira; Cribb Fabersunne, Camila; Yang, Ying-Kui; Kaelin, Christopher; Barsh, Gregory; Millhauser, Glenn. "Loop-swapped chimeras of the agouti-related protein and the agouti signaling protein identify contacts required for melanocortin 1 receptor selectivity and antagonism." J. Mol. Biol. 404, 45-55 (2010).
PubMed: 20831872

Assembly members:

Assembly members:
ASIP(93-126,_P103A,_P105A,_P111A,_Q115Y,_S124Y), polymer, 34 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ASIP(93-126,_P103A,_P105A,_P111A,_Q115Y,_S124Y): CVATRNSCKPAAAACCDPAA SCYCRFFRSACYCR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
spectral_peak_list
- spectral_peak_list_1

Data type	Count
1H chemical shifts	182

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ASIP(93-126, P103A, P105A, P111A, Q115Y, S124Y)	1

Entities:

Entity 1, ASIP(93-126, P103A, P105A, P111A, Q115Y, S124Y) 34 residues - Formula weight is not available

1

CYS

VAL

ALA

THR

ARG

ASN

SER

CYS

LYS

PRO

2

ALA

CYS

ASP

PRO

ALA

3

SER

CYS

TYR

CYS

ARG

PHE

ARG

SER

ALA

4

CYS

TYR

CYS

ARG

Samples:

sample_1: ASIP(93-126, P103A, P105A, P111A, Q115Y, S124Y)1 – 2 mM; D2O 10%; acetic acid, [U-100% 2H], 50 mM; sodium azide 0.1 w/v; TSP 200 uM; H2O 90%

sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

Varian INOVA 600 MHz