BMRB Entry 17086

Title:
1H assignments for ASIP(93-126, P103A, P105A, P111A, Q115Y, S124Y)
Deposition date:
2010-07-28
Original release date:
2011-10-28
Authors:
Patel, Mira; Cribb Fabersunne, Camila; Yang, Ying-kui; Kaelin, Chris; Barsh, Gregory; Millhauser, Glenn
Citation:

Citation: Patel, Mira; Cribb Fabersunne, Camila; Yang, Ying-Kui; Kaelin, Christopher; Barsh, Gregory; Millhauser, Glenn. "Loop-swapped chimeras of the agouti-related protein and the agouti signaling protein identify contacts required for melanocortin 1 receptor selectivity and antagonism."  J. Mol. Biol. 404, 45-55 (2010).
PubMed: 20831872

Assembly members:

Assembly members:
ASIP(93-126,_P103A,_P105A,_P111A,_Q115Y,_S124Y), polymer, 34 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ASIP(93-126,_P103A,_P105A,_P111A,_Q115Y,_S124Y): CVATRNSCKPAAAACCDPAA SCYCRFFRSACYCR

Data sets:
Data typeCount
1H chemical shifts182

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ASIP(93-126, P103A, P105A, P111A, Q115Y, S124Y)1

Entities:

Entity 1, ASIP(93-126, P103A, P105A, P111A, Q115Y, S124Y) 34 residues - Formula weight is not available

1   CYSVALALATHRARGASNSERCYSLYSPRO
2   ALAALAALAALACYSCYSASPPROALAALA
3   SERCYSTYRCYSARGPHEPHEARGSERALA
4   CYSTYRCYSARG

Samples:

sample_1: ASIP(93-126, P103A, P105A, P111A, Q115Y, S124Y)1 – 2 mM; D2O 10%; acetic acid, [U-100% 2H], 50 mM; sodium azide 0.1 w/v; TSP 200 uM; H2O 90%

sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Varian INOVA 600 MHz