BMRB Entry 16912

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16912
MolProbity Validation Chart

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Title:
Delta subunit of RNA polymerase from Bacillus subtilis
Deposition date:
2010-05-03
Original release date:
2010-10-05
Authors:
Motackova, Veronika; Novacek, Jiri; Zawadska-Kazimierczuk, Anna; Kazimierczuk, Krzysztof; Zidek, Lukas; Sanderova, Hana; Krasny, Libor; Kozminski, Wiktor; Sklenar, Vladimir
Citation:

Citation: Motakova, Veronika; Novaek, Jii; Zawadzka-Kazimierczuk, Anna; Kazimierczuk, Krzysztof; Zidek, Luka; Sanderova, Hana; Krasny, Libor; Komiski, Wiktor; Sklena, Vladimir. "Strategy for complete NMR assignment of disordered proteins with highly repetitive sequences based on resolution-enhanced 5D experiments."  J. Biomol. NMR 48, 169-177 (2010).
PubMed: 20890634

Assembly members:

Assembly members:
delta, polymer, 172 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
delta: GIKQYSQEELKEMALVEIAH ELFEEHKKPVPFQELLNEIA SLLGVKKEELGDRIAQFYTD LNIDGRFLALSDQTWGLRSW YPYDQLDEETQPTVKAKKKK AKKAVEEDLDLDEFEEIDED DLDLDEVEEELDLEADDFDE EDLDEDDDDLEIEEDIIDED DEDYDDEEEEIK

Data sets:
Data typeCount
13C chemical shifts752
15N chemical shifts183
1H chemical shifts1191

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1delta1

Entities:

Entity 1, delta 172 residues - Formula weight is not available

1   GLYILELYSGLNTYRSERGLNGLUGLULEU
2   LYSGLUMETALALEUVALGLUILEALAHIS
3   GLULEUPHEGLUGLUHISLYSLYSPROVAL
4   PROPHEGLNGLULEULEUASNGLUILEALA
5   SERLEULEUGLYVALLYSLYSGLUGLULEU
6   GLYASPARGILEALAGLNPHETYRTHRASP
7   LEUASNILEASPGLYARGPHELEUALALEU
8   SERASPGLNTHRTRPGLYLEUARGSERTRP
9   TYRPROTYRASPGLNLEUASPGLUGLUTHR
10   GLNPROTHRVALLYSALALYSLYSLYSLYS
11   ALALYSLYSALAVALGLUGLUASPLEUASP
12   LEUASPGLUPHEGLUGLUILEASPGLUASP
13   ASPLEUASPLEUASPGLUVALGLUGLUGLU
14   LEUASPLEUGLUALAASPASPPHEASPGLU
15   GLUASPLEUASPGLUASPASPASPASPLEU
16   GLUILEGLUGLUASPILEILEASPGLUASP
17   ASPGLUASPTYRASPASPGLUGLUGLUGLU
18   ILELYS

Samples:

sample_1: delta, [U-100% 13C; U-100% 15N], 0.7 mM; D2O, [U-2H], 10%; sodium chloride 10 mM; sodium azide 50 uM; phosphate buffer 20 mM; H2O 90%

sample_conditions_1: ionic strength: 30 mM; pH: 6.6; pressure: 1 atm; temperature: 301 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D CONsample_1isotropicsample_conditions_1
3D CBCACONsample_1isotropicsample_conditions_1
3D CBCANCOsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
5D HN_CA_CONHsample_1isotropicsample_conditions_1
5D HabCabCONHsample_1isotropicsample_conditions_1
5D H_CCtocsy_CONHsample_1isotropicsample_conditions_1

Software:

NMRPipe v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Varian Uniform NMR System 700 MHz

Related Database Links:

BMRB 16635 18903 19284
PDB
DBJ BAM55799 BAM59811
EMBL CAA89869 CAB15744 CCU60814 CEI59524 CEJ79381
GB AAA22710 ADM39706 ADV94526 AEP92774 AFI30331
REF NP_391597 WP_003221959 WP_003227609 WP_003235832 WP_003243661
SP P12464
AlphaFold P12464

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks