BMRB Entry 18903

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18903

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Title:
Relaxation study of Delta subunit of RNA polymerase from Bacillus subtilis
Deposition date:
2012-12-16
Original release date:
2014-02-14
Authors:
Kaderavek, Pavel; Sklenar, Vladimir; Zidek, Lukas; Rabatinova, Alzbeta; Krasny, Libor
Citation:

Citation: Kadeavek, Pavel; Zapletal, Vojtch; Rabatinova, Albeta; Krasny, Libor; Sklena, Vladimir; Zidek, Luka. "Spectral density mapping protocols for analysis of molecular motions in disordered proteins."  J. Biomol. NMR 58, 193-207 (2014).
PubMed: 24515886

Assembly members:

Assembly members:
delta, polymer, 172 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
delta: GIKQYSQEELKEMALVEIAH ELFEEHKKPVPFQELLNEIA SLLGVKKEELGDRIAQFYTD LNIDGRFLALSDQTWGLRSW YPYDQLDEETQPTVKAKKKK AKKAVEEDLDLDEFEEIDED DLDLDEVEEELDLEADDFDE EDLDEDDDDLEIEEDIIDED DEDYDDEEEEIK

Data sets:
Data typeCount
T1 relaxation values98
T2 relaxation values203
dipole-CSA cross correlation relaxation values264
heteronuclear NOE values96

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1delta1

Entities:

Entity 1, delta 172 residues - Formula weight is not available

1   GLYILELYSGLNTYRSERGLNGLUGLULEU
2   LYSGLUMETALALEUVALGLUILEALAHIS
3   GLULEUPHEGLUGLUHISLYSLYSPROVAL
4   PROPHEGLNGLULEULEUASNGLUILEALA
5   SERLEULEUGLYVALLYSLYSGLUGLULEU
6   GLYASPARGILEALAGLNPHETYRTHRASP
7   LEUASNILEASPGLYARGPHELEUALALEU
8   SERASPGLNTHRTRPGLYLEUARGSERTRP
9   TYRPROTYRASPGLNLEUASPGLUGLUTHR
10   GLNPROTHRVALLYSALALYSLYSLYSLYS
11   ALALYSLYSALAVALGLUGLUASPLEUASP
12   LEUASPGLUPHEGLUGLUILEASPGLUASP
13   ASPLEUASPLEUASPGLUVALGLUGLUGLU
14   LEUASPLEUGLUALAASPASPPHEASPGLU
15   GLUASPLEUASPGLUASPASPASPASPLEU
16   GLUILEGLUGLUASPILEILEASPGLUASP
17   ASPGLUASPTYRASPASPGLUGLUGLUGLU
18   ILELYS

Samples:

sample_1: delta, [U-100% 15N], 8.8 mg/mL; D2O, [U-2H], 10%; sodium chloride 10 mM; sodium azide 50 uM; phosphate buffer 20 mM; H2O 90%

sample_conditions_1: ionic strength: 30 mM; pH: 6.6; pressure: 1 atm; temperature: 300.15 K

Experiments:

NameSampleSample stateSample conditions
2D R1 15N,1H correlationsample_1isotropicsample_conditions_1
2D R2 15N,1H correlationsample_1isotropicsample_conditions_1
2D NOE 15N,1H correlationsample_1isotropicsample_conditions_1
2D 15N (coupled),1H correlationsample_1isotropicsample_conditions_1
2D 15N,1H correlation - transverse cross-correlated cross-relaxation (symmetrical reconversion)sample_1isotropicsample_conditions_1
2D 15N,1H correlation - longitudinal cross-correlated cross-relaxation (symmetrical reconversion)sample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

Relax, d'Auvergne, E. J. and Gooley, P. R. - data analysis

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz